The absence of N-acetylglucosamine in wall teichoic acids of Listeria monocytogenes modifies biofilm architecture and tolerance to rinsing and cleaning procedures

• Published in: PloS one Vol. 13; no. 1; p. e0190879
• Main Authors: Brauge, Thomas, Faille, Christine, Sadovskaya, Irina, Charbit, Alain, Benezech, Thierry, Shen, Yang, Loessner, Martin J, Bautista, Jean Romain, Midelet-Bourdin, Graziella
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.01.2018; Public Library of Science (PLoS)
• Subjects: Acetylglucosamine - metabolism; Acids; Adhesion; Architecture; Bacteria; Bacterial Adhesion - genetics; Bacterial Adhesion - physiology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biofilms; Biology and Life Sciences; Carbohydrates; Cell Wall - genetics; Cell Wall - metabolism; Cell Wall - ultrastructure; Cleaning; Clonal deletion; Colonies; Epidemics; Extracellular matrix; Food products; Food safety; Gene deletion; Gene mutation; Genes; Genetic aspects; Hydrophobic and Hydrophilic Interactions; Laboratories; Life Sciences; Listeria; Listeria monocytogenes; Listeria monocytogenes - genetics; Listeria monocytogenes - physiology; Listeria monocytogenes - ultrastructure; Medicine and Health Sciences; Microbiology and Parasitology; Microscopy, Electron, Transmission; Mutagenesis; Mutants; Mutation; N-Acetylglucosamine; Nutrition; Phenotype; Physical Sciences; Point mutation; Seafood; Shear stress; Splicing; Stainless Steel; Stainless steels; Stress, Mechanical; Structural steels; Teichoic acids; Teichoic Acids - metabolism; Water; Water flow; Biofilm; Force d'interaction; Fraction saccharidique; Listeria; Acide téichoïque
• DOI: 10.1371/journal.pone.0190879
• ISSN: 1932-6203

The wall teichoic acid (WTA) is the major carbohydrate found within the extracellular matrix of the Listeria monocytogenes biofilm. We first addressed the frequency of spontaneous mutations in two genes (lmo2549 and lmo2550) responsible for the GlcNAcylation in 93 serotype 1/2a strains that were mainly isolated from seafood industries. We studied the impact of mutations in lmo2549 or lmo2550 genes on biofilm formation by using one mutant carrying a natural mutation inactivating the lmo2550 gene (DSS 1130 BFA2 strain) and two EGD-e mutants that lack respective genes by in-frame deletion of lmo2549 or lmo2550 using splicing-by-overlap-extension PCR, followed by allelic exchange mutagenesis. The lmo2550 gene mutation, occurring in around 50% isolates, caused a decrease in bacterial adhesion to stainless steel compared to wild-type EGD-e strain during the adhesion step. On the other hand, bacterial population weren't significantly different after 24h-biofilm formation. The biofilm architecture was different between the wild-type strain and the two mutants inactivated for lmo2549 or lmo2550 genes respectively with the presence of bacterial micro-colonies for mutants which were not observed in the wild-type EGD-e strain biofilm. These differences might account for the stronger hydrophilic surface exhibited by the mutant cells. Upon a water flow or to a cleaning procedure at a shear stress of 0.16 Pa, the mutant biofilms showed the higher detachment rate compared to wild-type strain. Meanwhile, an increase in the amount of residual viable but non-culturable population on stainless steel was recorded in two mutants. Our data suggests that the GlcNAc residue of WTA played a role in adhesion and biofilm formation of Listeria monocyctogenes.