Evaluating Cellular Polyfunctionality with a Novel Polyfunctionality Index

• Published in: PloS one Vol. 7; no. 7; p. e42403
• Main Authors: Larsen, Martin, Sauce, Delphine, Arnaud, Laurent, Fastenackels, Solène, Appay, Victor, Gorochov, Guy
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.07.2012; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Arthritis; Biology; Charts; Chemokines; Cluster analysis; Combinatorial analysis; Cytokines; Cytometry; Data analysis; Datasets; Demand analysis; Flow Cytometry; HIV; Human immunodeficiency virus; Humans; Immunology; Interferon; Life Sciences; Lymphocytes; Lymphocytes T; Medicine; Monkeys; Species Specificity; Statistical analysis; Statistical tests; Studies; T cell receptors; T cells; T-Lymphocytes - cytology; Vaccination; γ-Interferon; France

Functional evaluation of naturally occurring or vaccination-induced T cell responses in mice, men and monkeys has in recent years advanced from single-parameter (e.g. IFN-γ-secretion) to much more complex multidimensional measurements. Co-secretion of multiple functional molecules (such as cytokines...