A novel membrane complex is required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila

In the ciliate Tetrahymena thermophila , lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extens...

Full description

Saved in:
Bibliographic Details
Published inPLoS genetics Vol. 18; no. 5; p. e1010194
Main Authors Kuppannan, Aarthi, Jiang, Yu-Yang, Maier, Wolfgang, Liu, Chang, Lang, Charles F., Cheng, Chao-Yin, Field, Mark C., Zhao, Minglei, Zoltner, Martin, Turkewitz, Aaron P.
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 19.05.2022
Public Library of Science (PLoS)
Subjects
Animals
Biology and Life Sciences
Cell membranes
Ciliates
Defects
Exocytosis
Exocytosis - genetics
Genetic aspects
Localization
Lysosomes
Lysosomes - metabolism
Macromolecules
Mutation
Organelles
Organelles - metabolism
Physiological aspects
Plasma
Proteins
Research and Analysis Methods
Secretory Vesicles - genetics
Secretory Vesicles - metabolism
Signal transduction
Tetrahymena
Tetrahymena thermophila
Tetrahymena thermophila - genetics
United States
Online AccessGet full text

Cover

Abstract In the ciliate Tetrahymena thermophila , lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extensively described in animals but it is not clear whether similar mechanisms exist in ciliates or their sister lineage, the Apicomplexan parasites, which together belong to the ecologically and medically important superphylum Alveolata. Beginning with a T . thermophila mutant in mucocyst exocytosis, we used a forward genetic approach to uncover MDL1 ( M ucocyst D ischarge with a L amG domain), a novel gene that is essential for regulated exocytosis of mucocysts. Mdl1p is a 40 kDa membrane glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. This sub-localization of Mdl1p, which occurs prior to docking, underscores a functional asymmetry in mucocysts that is strikingly similar to that of highly polarized secretory organelles in other Alveolates. A mis-sense mutation in the LamG domain results in mucocysts that dock but only undergo inefficient exocytosis. In contrast, complete knockout of MDL1 largely prevents mucocyst docking itself. Mdl1p is physically associated with 9 other proteins, all of them novel and largely restricted to Alveolates, and sedimentation analysis supports the idea that they form a large complex. Analysis of three other members of this putative complex, called MDD (for M ucocyst D ocking and D ischarge), shows that they also localize to mucocysts. Negative staining of purified MDD complexes revealed distinct particles with a central channel. Our results uncover a novel macromolecular complex whose subunits are conserved within alveolates but not in other lineages, that is essential for regulated exocytosis in T . thermophila .
AbstractList In the ciliate Tetrahymena thermophila, lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extensively described in animals but it is not clear whether similar mechanisms exist in ciliates or their sister lineage, the Apicomplexan parasites, which together belong to the ecologically and medically important superphylum Alveolata. Beginning with a T. thermophila mutant in mucocyst exocytosis, we used a forward genetic approach to uncover MDL1 (Mucocyst Discharge with a LamG domain), a novel gene that is essential for regulated exocytosis of mucocysts. Mdl1p is a 40 kDa membrane glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. This sub-localization of Mdl1p, which occurs prior to docking, underscores a functional asymmetry in mucocysts that is strikingly similar to that of highly polarized secretory organelles in other Alveolates. A mis-sense mutation in the LamG domain results in mucocysts that dock but only undergo inefficient exocytosis. In contrast, complete knockout of MDL1 largely prevents mucocyst docking itself. Mdl1p is physically associated with 9 other proteins, all of them novel and largely restricted to Alveolates, and sedimentation analysis supports the idea that they form a large complex. Analysis of three other members of this putative complex, called MDD (for Mucocyst Docking and Discharge), shows that they also localize to mucocysts. Negative staining of purified MDD complexes revealed distinct particles with a central channel. Our results uncover a novel macromolecular complex whose subunits are conserved within alveolates but not in other lineages, that is essential for regulated exocytosis in T. thermophila.
In the ciliate Tetrahymena thermophila , lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extensively described in animals but it is not clear whether similar mechanisms exist in ciliates or their sister lineage, the Apicomplexan parasites, which together belong to the ecologically and medically important superphylum Alveolata. Beginning with a T . thermophila mutant in mucocyst exocytosis, we used a forward genetic approach to uncover MDL1 ( M ucocyst D ischarge with a L amG domain), a novel gene that is essential for regulated exocytosis of mucocysts. Mdl1p is a 40 kDa membrane glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. This sub-localization of Mdl1p, which occurs prior to docking, underscores a functional asymmetry in mucocysts that is strikingly similar to that of highly polarized secretory organelles in other Alveolates. A mis-sense mutation in the LamG domain results in mucocysts that dock but only undergo inefficient exocytosis. In contrast, complete knockout of MDL1 largely prevents mucocyst docking itself. Mdl1p is physically associated with 9 other proteins, all of them novel and largely restricted to Alveolates, and sedimentation analysis supports the idea that they form a large complex. Analysis of three other members of this putative complex, called MDD (for M ucocyst D ocking and D ischarge), shows that they also localize to mucocysts. Negative staining of purified MDD complexes revealed distinct particles with a central channel. Our results uncover a novel macromolecular complex whose subunits are conserved within alveolates but not in other lineages, that is essential for regulated exocytosis in T . thermophila . All cells, whether single-celled protists or multicellular organisms, interact dynamically with their environments. One important mode of interaction is the release of molecules, a phenomenon called secretion, which can then modify the environment to promote the organism’s well-being. Moreover, many cells have the capacity to rapidly adjust the pathways that underlie secretion, allowing them to tailor their secretory behavior in response to changes in their surroundings. A dramatic example of this is the capacity to synthesize and then store reservoirs of secretory molecules, whose eventual release is triggered when the cell senses specific environmental conditions. This phenomenon is called ‘regulated exocytosis’ and has been long studied in animals, because it serves as the basis for communication between different cells and tissues. Many single-celled organisms can also secrete via regulated exocytosis, and understanding the mechanisms involved could have practical consequences for developing therapies against several devastating human parasites. In this paper, we took a genetic approach to identifying factors involved in exocytosis in a single-celled protist, the ciliate Tetrahymena thermophila . We find that a novel gene, which appears to be present only in Tetrahymena and its relatively close evolutionary relatives, plays an important role in the pathway. Our results add another layer to recent findings that cells like Tetrahymena evolved unique mechanisms for regulated exocytosis, expanding our appreciation of cellular biodiversity.
In the ciliate Tetrahymena thermophila, lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extensively described in animals but it is not clear whether similar mechanisms exist in ciliates or their sister lineage, the Apicomplexan parasites, which together belong to the ecologically and medically important superphylum Alveolata. Beginning with a T. thermophila mutant in mucocyst exocytosis, we used a forward genetic approach to uncover MDL1 (Mucocyst Discharge with a LamG domain), a novel gene that is essential for regulated exocytosis of mucocysts. Mdl1p is a 40 kDa membrane glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. This sub-localization of Mdl1p, which occurs prior to docking, underscores a functional asymmetry in mucocysts that is strikingly similar to that of highly polarized secretory organelles in other Alveolates. A mis-sense mutation in the LamG domain results in mucocysts that dock but only undergo inefficient exocytosis. In contrast, complete knockout of MDL1 largely prevents mucocyst docking itself. Mdl1p is physically associated with 9 other proteins, all of them novel and largely restricted to Alveolates, and sedimentation analysis supports the idea that they form a large complex. Analysis of three other members of this putative complex, called MDD (for Mucocyst Docking and Discharge), shows that they also localize to mucocysts. Negative staining of purified MDD complexes revealed distinct particles with a central channel. Our results uncover a novel macromolecular complex whose subunits are conserved within alveolates but not in other lineages, that is essential for regulated exocytosis in T. thermophila.In the ciliate Tetrahymena thermophila, lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extensively described in animals but it is not clear whether similar mechanisms exist in ciliates or their sister lineage, the Apicomplexan parasites, which together belong to the ecologically and medically important superphylum Alveolata. Beginning with a T. thermophila mutant in mucocyst exocytosis, we used a forward genetic approach to uncover MDL1 (Mucocyst Discharge with a LamG domain), a novel gene that is essential for regulated exocytosis of mucocysts. Mdl1p is a 40 kDa membrane glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. This sub-localization of Mdl1p, which occurs prior to docking, underscores a functional asymmetry in mucocysts that is strikingly similar to that of highly polarized secretory organelles in other Alveolates. A mis-sense mutation in the LamG domain results in mucocysts that dock but only undergo inefficient exocytosis. In contrast, complete knockout of MDL1 largely prevents mucocyst docking itself. Mdl1p is physically associated with 9 other proteins, all of them novel and largely restricted to Alveolates, and sedimentation analysis supports the idea that they form a large complex. Analysis of three other members of this putative complex, called MDD (for Mucocyst Docking and Discharge), shows that they also localize to mucocysts. Negative staining of purified MDD complexes revealed distinct particles with a central channel. Our results uncover a novel macromolecular complex whose subunits are conserved within alveolates but not in other lineages, that is essential for regulated exocytosis in T. thermophila.
In the ciliate Tetrahymena thermophila , lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in response to extracellular events, a phenomenon called regulated exocytosis. The molecular bases underlying regulated exocytosis have been extensively described in animals but it is not clear whether similar mechanisms exist in ciliates or their sister lineage, the Apicomplexan parasites, which together belong to the ecologically and medically important superphylum Alveolata. Beginning with a T . thermophila mutant in mucocyst exocytosis, we used a forward genetic approach to uncover MDL1 ( M ucocyst D ischarge with a L amG domain), a novel gene that is essential for regulated exocytosis of mucocysts. Mdl1p is a 40 kDa membrane glycoprotein that localizes to mucocysts, and specifically to a tip domain that contacts the plasma membrane when the mucocyst is docked. This sub-localization of Mdl1p, which occurs prior to docking, underscores a functional asymmetry in mucocysts that is strikingly similar to that of highly polarized secretory organelles in other Alveolates. A mis-sense mutation in the LamG domain results in mucocysts that dock but only undergo inefficient exocytosis. In contrast, complete knockout of MDL1 largely prevents mucocyst docking itself. Mdl1p is physically associated with 9 other proteins, all of them novel and largely restricted to Alveolates, and sedimentation analysis supports the idea that they form a large complex. Analysis of three other members of this putative complex, called MDD (for M ucocyst D ocking and D ischarge), shows that they also localize to mucocysts. Negative staining of purified MDD complexes revealed distinct particles with a central channel. Our results uncover a novel macromolecular complex whose subunits are conserved within alveolates but not in other lineages, that is essential for regulated exocytosis in T . thermophila .
Audience Academic
Author Zhao, Minglei
Zoltner, Martin
Turkewitz, Aaron P.
Lang, Charles F.
Kuppannan, Aarthi
Jiang, Yu-Yang
Field, Mark C.
Cheng, Chao-Yin
Maier, Wolfgang
Liu, Chang
AuthorAffiliation 2 Bio3/Bioinformatics and Molecular Genetics, Faculty of Biology and ZBMZ, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany
6 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic
5 School of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom
7 Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (BIOCEV), Vestec, Czech Republic
1 Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United State of America
University of Virginia, UNITED STATES
4 Committee on Genetics, Genomics, and Systems Biology, The University of Chicago, Chicago, Illinois, United States of America
3 Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, United States of America
AuthorAffiliation_xml – name: 5 School of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom
– name: 2 Bio3/Bioinformatics and Molecular Genetics, Faculty of Biology and ZBMZ, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany
– name: 1 Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois, United State of America
– name: 3 Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, United States of America
– name: 7 Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (BIOCEV), Vestec, Czech Republic
– name: 4 Committee on Genetics, Genomics, and Systems Biology, The University of Chicago, Chicago, Illinois, United States of America
– name: 6 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic
– name: University of Virginia, UNITED STATES
Author_xml – sequence: 1
  givenname: Aarthi
  orcidid: 0000-0002-0079-2402
  surname: Kuppannan
  fullname: Kuppannan, Aarthi
– sequence: 2
  givenname: Yu-Yang
  orcidid: 0000-0002-0338-4252
  surname: Jiang
  fullname: Jiang, Yu-Yang
– sequence: 3
  givenname: Wolfgang
  orcidid: 0000-0002-9464-6640
  surname: Maier
  fullname: Maier, Wolfgang
– sequence: 4
  givenname: Chang
  orcidid: 0000-0001-9373-8458
  surname: Liu
  fullname: Liu, Chang
– sequence: 5
  givenname: Charles F.
  orcidid: 0000-0001-6428-3941
  surname: Lang
  fullname: Lang, Charles F.
– sequence: 6
  givenname: Chao-Yin
  orcidid: 0000-0002-9530-524X
  surname: Cheng
  fullname: Cheng, Chao-Yin
– sequence: 7
  givenname: Mark C.
  orcidid: 0000-0002-4866-2885
  surname: Field
  fullname: Field, Mark C.
– sequence: 8
  givenname: Minglei
  orcidid: 0000-0001-5832-6060
  surname: Zhao
  fullname: Zhao, Minglei
– sequence: 9
  givenname: Martin
  orcidid: 0000-0002-0214-285X
  surname: Zoltner
  fullname: Zoltner, Martin
– sequence: 10
  givenname: Aaron P.
  orcidid: 0000-0003-3531-5806
  surname: Turkewitz
  fullname: Turkewitz, Aaron P.
BackLink https://www.ncbi.nlm.nih.gov/pubmed/35587496$$D View this record in MEDLINE/PubMed
BookMark eNqVk1tv0zAUxyM0xC7wDRBEQkLw0JLETtzsAamauFSamASDV8uxTxIPx-7sZGqf-eKc0m5qpgmB_GDL53f-52Kf4-jAOgtR9DxNpilh6bsrN3grzHTZgJ2mCa6SPoqO0jwnE0YTerB3PoyOQ7hKEpLPSvYkOiR5PmO0LI6iX_PYuhswcQdd5YWFWLpuaWAV6xB7uB60BxXXzsfKyZ_aNrGwCg3NYESPFlg5ue5dQNrVsVkHF1wHEw9bs_MNahoDIdY2voTei3bdgRVx34Lv3LLVRjyNHtfCBHi220-i7x8_XJ59npxffFqczc8nkmVFPylVUhNaCUgyRWhRUqkqoeoZCMaAzVShGMmoEhJUKYTIMlpBriTQUuV1TSpyEr3c6i6NC3zXv8CzgrGCZCnLkVhsCeXEFV963Qm_5k5o_ucCq-HC91oa4AUt8hmkrKwLSjMKFSWSJiRjUKUkrTPUer-LNlQdYB4Wizcj0bHF6pY37oaXaV5iPijwZifg3fUAoeedDhKbiR11wybvoiiTWVFu0Ff30Ier21GNwAK0rR3GlRtRPmdJzliOckhNH6BwKei0xD9Ya7wfObwdOSDTw6pvxBACX3z7-h_sl39nL36M2dd7bAvC9G1wZui1s2EMvth_lbvnuJ0IBOgWkN6F4KG-Q9KEbwbvtrV8M3h8N3jodnrPTepebMJj97T5u_Nv7r82QQ
CitedBy_id crossref_primary_10_1083_jcb_202307047
crossref_primary_10_1016_j_isci_2024_111123
crossref_primary_10_1016_j_ceb_2022_102151
crossref_primary_10_1016_j_celrep_2024_113740
crossref_primary_10_1016_j_protis_2022_125909
Cites_doi 10.1093/molbev/mss060
10.1186/1472-6750-6-19
10.1016/j.jbior.2021.100807
10.1242/jcs.178285
10.1111/j.1600-0854.2005.00273.x
10.1534/genetics.117.200766
10.4161/cam.21831
10.1111/tra.12646
10.1111/j.1600-0854.2007.00548.x
10.1242/jcs.111.1.131
10.1093/database/bat008
10.1091/mbc.e05-01-0028
10.1111/mmi.14674
10.1111/tra.12450
10.1016/j.ejcb.2011.06.002
10.1002/cbin.10337
10.7554/eLife.19090
10.1038/s41467-019-11979-z
10.1152/physrev.00031.2002
10.1083/jcb.139.5.1197
10.1016/j.chom.2012.03.006
10.1126/sciadv.1602552
10.1016/S0300-9084(00)00201-7
10.1093/nar/gkj054
10.1073/pnas.2026811118
10.1111/j.1550-7408.2003.tb00260.x
10.1128/EC.4.12.2129-2139.2005
10.1093/genetics/146.1.135
10.1046/j.1600-0854.2003.00155.x
10.1016/j.ceb.2019.05.003
10.1038/nature11320
10.1016/j.mimet.2010.07.009
10.1111/j.1550-7408.1970.tb02366.x
10.1242/jcs.64.1.49
10.1038/s41564-019-0563-x
10.1002/jez.1402600111
10.3389/fmicb.2015.01313
10.1080/19420862.2016.1228504
10.1101/cshperspect.a009712
10.1016/S0167-4889(03)00092-2
10.1016/0003-2697(89)90115-2
10.1093/genetics/157.2.611
10.1091/mbc.e17-01-0018
10.1242/jcs.238659
10.1091/mbc.9.2.497
10.1016/j.ceb.2013.04.008
10.1111/j.1550-7408.2005.00045.x
10.1074/mcp.M113.031591
10.1016/j.cub.2018.01.047
10.1111/cmi.13018
10.1016/j.sbi.2018.11.007
10.1038/nbt.1511
10.1083/jcb.135.6.1775
10.1091/mbc.e12-05-0369
10.1038/srep33555
10.1038/nmeth.1923
10.1074/jbc.M117.816884
10.1016/S0091-679X(08)61535-6
10.1016/j.ijmm.2018.07.007
10.4049/jimmunol.1800268
10.1002/j.1460-2075.1991.tb07727.x
10.1016/j.ceb.2007.05.001
10.1091/mbc.8.6.1063
10.1016/j.ejop.2017.04.001
10.1083/jcb.69.1.126
10.3389/fcell.2019.00195
10.1038/nmeth.3901
10.1038/nrm2745
10.1083/jcb.201305086
10.1017/S0031182015001213
10.4161/fly.19695
10.1016/S0091-679X(08)61528-9
10.1111/j.1600-0854.2007.00544.x
10.1111/jeu.12106
10.1016/0169-4758(92)90265-4
10.1101/cshperspect.a016840
10.1128/EC.00058-15
10.1016/j.devcel.2020.10.020
10.1371/journal.pone.0004429
10.1093/genetics/159.4.1605
10.7554/eLife.42166
10.1128/mSphere.00027-17
10.1534/genetics.118.301889
10.1016/j.bbamcr.2021.118947
10.1111/cmi.12426
10.1016/j.protis.2018.03.004
10.1016/S0091-679X(08)61530-7
10.4049/jimmunol.1800426
10.1111/j.1600-0854.2004.00194.x
10.1186/gb-2011-12-10-r100
10.1242/jcs.208033
10.1042/BST0330652
10.1371/journal.pbio.0040286
10.1016/j.gene.2008.08.007
10.1093/nar/gky1106
10.1093/nar/gky379
10.1007/978-1-4939-3530-7_3
ContentType Journal Article
Copyright COPYRIGHT 2022 Public Library of Science
2022 Kuppannan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2022 Kuppannan et al 2022 Kuppannan et al
Copyright_xml – notice: COPYRIGHT 2022 Public Library of Science
– notice: 2022 Kuppannan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2022 Kuppannan et al 2022 Kuppannan et al
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
IOV
ISN
ISR
3V.
7QP
7QR
7SS
7TK
7TM
7TO
7X7
7XB
88E
8FD
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FR3
FYUFA
GHDGH
GNUQQ
H94
HCIFZ
K9.
LK8
M0S
M1P
M7P
P64
PHGZM
PHGZT
PIMPY
PJZUB
PKEHL
PPXIY
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
RC3
7X8
5PM
DOA
DOI 10.1371/journal.pgen.1010194
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Gale In Context: Opposing Viewpoints
Gale In Context: Canada
Gale In Context: Science
ProQuest Central (Corporate)
Calcium & Calcified Tissue Abstracts
Chemoreception Abstracts
Entomology Abstracts (Full archive)
Neurosciences Abstracts
Nucleic Acids Abstracts
Oncogenes and Growth Factors Abstracts
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central
Engineering Research Database
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
AIDS and Cancer Research Abstracts
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
ProQuest Health & Medical Collection
Medical Database
Biological Science Database
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest Health & Medical Research Collection
ProQuest One Academic Middle East (New)
ProQuest One Health & Nursing
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest Central Student
Oncogenes and Growth Factors Abstracts
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
Nucleic Acids Abstracts
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest One Health & Nursing
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
ProQuest One Applied & Life Sciences
ProQuest Health & Medical Research Collection
Genetics Abstracts
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Health & Medical Research Collection
Biological Science Collection
AIDS and Cancer Research Abstracts
Chemoreception Abstracts
ProQuest Central (New)
ProQuest Medical Library (Alumni)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
Neurosciences Abstracts
ProQuest Hospital Collection (Alumni)
Biotechnology and BioEngineering Abstracts
Entomology Abstracts
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
Calcium & Calcified Tissue Abstracts
ProQuest One Academic (New)
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database




MEDLINE

MEDLINE - Academic
CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
DocumentTitleAlternate An integral membrane complex localized to mucocyst tips is required for docking and exocytic fusion
EISSN 1553-7404
ExternalDocumentID 2677632175
oai_doaj_org_article_64658e179f64424eb43c40327eb131f2
PMC9159632
A705775908
35587496
10_1371_journal_pgen_1010194
Genre Research Support, Non-U.S. Gov't
Journal Article
Research Support, N.I.H., Extramural
GeographicLocations United States
GeographicLocations_xml – name: United States
GrantInformation_xml – fundername: Wellcome Trust
  grantid: 204697/Z/16/Z
– fundername: NIGMS NIH HHS
  grantid: T32 GM007197
– fundername: NIGMS NIH HHS
  grantid: R35 GM143052
– fundername: NIGMS NIH HHS
  grantid: R01 GM105783
– fundername: ;
  grantid: 031L0101C de.NBI-epi
– fundername: ;
  grantid: 204697/Z/16/Z
– fundername: ;
  grantid: OPVVV/0000759
– fundername: ;
  grantid: R01GM105783
– fundername: ;
  grantid: T32 GM007197
GroupedDBID ---
123
29O
2WC
53G
5VS
7X7
88E
8FE
8FH
8FI
8FJ
AAFWJ
AAUCC
AAWOE
AAYXX
ABDBF
ABUWG
ACGFO
ACIHN
ACIWK
ACPRK
ACUHS
ADBBV
AEAQA
AENEX
AFKRA
AFPKN
AHMBA
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
B0M
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
BWKFM
CCPQU
CITATION
CS3
DIK
DU5
E3Z
EAP
EAS
EBD
EBS
EJD
EMK
EMOBN
ESX
F5P
FPL
FYUFA
GROUPED_DOAJ
GX1
HCIFZ
HMCUK
HYE
IAO
IGS
IHR
IHW
INH
INR
IOV
ISN
ISR
ITC
KQ8
LK8
M1P
M48
M7P
O5R
O5S
OK1
OVT
P2P
PHGZM
PHGZT
PIMPY
PQQKQ
PROAC
PSQYO
PV9
QF4
QN7
RNS
RPM
RZL
SV3
TR2
TUS
UKHRP
WOW
XSB
~8M
ADRAZ
C1A
CGR
CUY
CVF
ECM
EIF
H13
IPNFZ
NPM
PJZUB
PPXIY
PQGLB
RIG
WOQ
PMFND
3V.
7QP
7QR
7SS
7TK
7TM
7TO
7XB
8FD
8FK
AZQEC
DWQXO
FR3
GNUQQ
H94
K9.
P64
PKEHL
PQEST
PQUKI
PRINS
RC3
7X8
5PM
PUEGO
-
AAPBV
ABPTK
ACDSR
ADACO
BBAFP
M~E
ID FETCH-LOGICAL-c726t-9d0f34bae02d34694cdbadf8ea77e78d6d7324daced9aaa224be5dce49d5ff3b3
IEDL.DBID 7X7
ISSN 1553-7404
1553-7390
IngestDate Sun Jul 03 03:47:33 EDT 2022
Wed Aug 27 01:22:47 EDT 2025
Thu Aug 21 18:19:44 EDT 2025
Fri Jul 11 03:49:30 EDT 2025
Fri Jul 25 12:12:44 EDT 2025
Tue Jun 17 21:24:49 EDT 2025
Tue Jun 10 20:31:49 EDT 2025
Fri Jun 27 05:03:41 EDT 2025
Fri Jun 27 04:09:17 EDT 2025
Fri Jun 27 03:52:35 EDT 2025
Thu May 22 21:20:26 EDT 2025
Mon Jul 21 06:03:42 EDT 2025
Tue Jul 01 04:14:58 EDT 2025
Thu Apr 24 22:58:54 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 5
Language English
License This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Creative Commons Attribution License
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c726t-9d0f34bae02d34694cdbadf8ea77e78d6d7324daced9aaa224be5dce49d5ff3b3
Notes new_version
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors share first authorship on this work.
The authors have declared that no competing interests exist.
ORCID 0000-0002-0079-2402
0000-0002-0338-4252
0000-0001-9373-8458
0000-0002-9464-6640
0000-0002-9530-524X
0000-0003-3531-5806
0000-0001-6428-3941
0000-0002-0214-285X
0000-0002-4866-2885
0000-0001-5832-6060
OpenAccessLink https://www.proquest.com/docview/2677632175?pq-origsite=%requestingapplication%
PMID 35587496
PQID 2677632175
PQPubID 1436339
PageCount e1010194
ParticipantIDs plos_journals_2677632175
doaj_primary_oai_doaj_org_article_64658e179f64424eb43c40327eb131f2
pubmedcentral_primary_oai_pubmedcentral_nih_gov_9159632
proquest_miscellaneous_2666908692
proquest_journals_2677632175
gale_infotracmisc_A705775908
gale_infotracacademiconefile_A705775908
gale_incontextgauss_ISR_A705775908
gale_incontextgauss_ISN_A705775908
gale_incontextgauss_IOV_A705775908
gale_healthsolutions_A705775908
pubmed_primary_35587496
crossref_primary_10_1371_journal_pgen_1010194
crossref_citationtrail_10_1371_journal_pgen_1010194
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2022-05-19
PublicationDateYYYYMMDD 2022-05-19
PublicationDate_xml – month: 05
  year: 2022
  text: 2022-05-19
  day: 19
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: San Francisco
– name: San Francisco, CA USA
PublicationTitle PLoS genetics
PublicationTitleAlternate PLoS Genet
PublicationYear 2022
Publisher Public Library of Science
Public Library of Science (PLoS)
Publisher_xml – name: Public Library of Science
– name: Public Library of Science (PLoS)
References P. Saftig (pgen.1010194.ref002) 2009; 10
L. Le (pgen.1010194.ref007) 2021
H. Plattner (pgen.1010194.ref082) 2010; 280
D. Sparvoli (pgen.1010194.ref099) 2020; 133
Y. Miyatake (pgen.1010194.ref045) 2018; 201
T. Harumoto (pgen.1010194.ref033) 1991; 260
E. Wyroba (pgen.1010194.ref085) 1995; 68
E. Afgan (pgen.1010194.ref091) 2018; 46
E. Aquilini (pgen.1010194.ref044) 2021
J.W. Verbsky (pgen.1010194.ref079) 1998; 9
S. Kumar (pgen.1010194.ref061) 2014
F. Buonanno (pgen.1010194.ref031) 2014; 61
E.P. Hamilton (pgen.1010194.ref094) 2016; 5
L. Liu (pgen.1010194.ref083) 2011; 90
L. Vayssie (pgen.1010194.ref054) 2000; 82
J. Calow (pgen.1010194.ref078) 2016; 8
J.P. Luzio (pgen.1010194.ref001) 2014; 6
E. Orias (pgen.1010194.ref088) 2000; 62
T. Ota (pgen.1010194.ref105) 2018; 169
S. Schmollinger (pgen.1010194.ref019) 2021; 118
R. Docampo (pgen.1010194.ref020) 2021; 1868
A.T. Cowan (pgen.1010194.ref052) 2005; 16
J.A. Eisen (pgen.1010194.ref092) 2006; 4
G. Rosati (pgen.1010194.ref030) 2003; 50
J.C. Adams (pgen.1010194.ref073) 2011; 3
A. Gimmler (pgen.1010194.ref025) 2016; 6
E. Orias (pgen.1010194.ref087) 2000; 62
Y.Y. Jiang (pgen.1010194.ref060) 2017; 206
S. Guerrier (pgen.1010194.ref051) 2017; 18
S. Kumar (pgen.1010194.ref062) 2015; 14
E. Hohenester (pgen.1010194.ref076) 2013; 7
D. Gogendeau (pgen.1010194.ref041) 2005; 4
K. Venugopal (pgen.1010194.ref024) 2018; 308
P.J. Sloves (pgen.1010194.ref036) 2012; 11
A.P. Turkewitz (pgen.1010194.ref072) 1991; 10
K. Mochizuki (pgen.1010194.ref095) 2008; 425
T.J. Templeton (pgen.1010194.ref021) 2016; 143
J. Cox (pgen.1010194.ref100) 2014; 13
G.R. Bowman (pgen.1010194.ref049) 2005; 6
G.R. Bowman (pgen.1010194.ref058) 2001; 159
F. Brozzi (pgen.1010194.ref013) 2012; 23
T. Weisse (pgen.1010194.ref026) 2017; 61
B. Langmead (pgen.1010194.ref089) 2012; 9
P. Cingolani (pgen.1010194.ref093) 2012; 6
C.J. Ma (pgen.1010194.ref010) 2021; 80
E. Orias (pgen.1010194.ref047) 1983; 64
M.B. Khawar (pgen.1010194.ref006) 2019; 7
N.M. Chasen (pgen.1010194.ref069) 2017; 2
D. Sparvoli (pgen.1010194.ref038) 2018; 28
D.J. Dubois (pgen.1010194.ref023) 2019; 21
Y.Y. Jiang (pgen.1010194.ref090) 2019; 211
J. Morlon-Guyot (pgen.1010194.ref037) 2015; 17
E. Hohenester (pgen.1010194.ref075) 2019; 56
B.H. Satir (pgen.1010194.ref084) 2015; 39
F. Fan (pgen.1010194.ref016) 2017; 6
H. Wessenberg (pgen.1010194.ref032) 1970; 17
N.R. Bin (pgen.1010194.ref017) 2018; 201
Y. Perez-Riverol (pgen.1010194.ref104) 2019; 47
R. Ben Chaabene (pgen.1010194.ref022) 2021; 115
R.D. Burgoyne (pgen.1010194.ref009) 2003; 83
T. Weide (pgen.1010194.ref077) 2006; 6
G.S. Gavelis (pgen.1010194.ref055) 2017; 3
H. Plattner (pgen.1010194.ref056) 2003; 1641
M. Froissard (pgen.1010194.ref039) 2001; 157
J. Xiong (pgen.1010194.ref065) 2013; 2013
D. Cassidy-Hanley (pgen.1010194.ref097) 1997; 146
S.O. Obado (pgen.1010194.ref098) 2016; 1411
G. Espinoza-Vergara (pgen.1010194.ref027) 2019; 4
M.S. Ewing (pgen.1010194.ref034) 1992; 8
J. Beisson (pgen.1010194.ref043) 1976; 69
H. Kaur (pgen.1010194.ref063) 2017; 28
K. Kataoka (pgen.1010194.ref096) 2010; 82
S.L. Bowman (pgen.1010194.ref011) 2019; 20
C.J. Newbold (pgen.1010194.ref029) 2015; 6
N.D. Chilcoat (pgen.1010194.ref059) 1996; 135
B.R. Ali (pgen.1010194.ref012) 2005; 33
R.S. Coyne (pgen.1010194.ref028) 2011; 12
L. Meng (pgen.1010194.ref074) 2020; 55
B. Fahey (pgen.1010194.ref066) 2012; 29
S. Tyanova (pgen.1010194.ref103) 2016; 13
E.M. Rodarte (pgen.1010194.ref018) 2018; 293
I.L. Conte (pgen.1010194.ref014) 2016; 129
V.S. Lopes (pgen.1010194.ref015) 2007; 8
F. Maley (pgen.1010194.ref068) 1989; 180
M.S. Marks (pgen.1010194.ref003) 2013; 25
G.R. Bowman (pgen.1010194.ref053) 2005; 52
W. Miao (pgen.1010194.ref064) 2009; 4
C. Delevoye (pgen.1010194.ref005) 2019; 59
A. Rahaman (pgen.1010194.ref057) 2009
G. Raposo (pgen.1010194.ref004) 2007; 19
R. Kissmehl (pgen.1010194.ref081) 2007; 8
S.M. Melia (pgen.1010194.ref048) 1998; 111
M. Froissard (pgen.1010194.ref040) 2004; 5
J. Cox (pgen.1010194.ref101) 2008; 26
A.P. Turkewitz (pgen.1010194.ref050) 2004; 5
C. Kontur (pgen.1010194.ref080) 2016
C. Suarez (pgen.1010194.ref046) 2019; 10
R. Jahn (pgen.1010194.ref071) 2012; 490
J.J. McCormack (pgen.1010194.ref008) 2017; 130
N.D. Chilcoat (pgen.1010194.ref086) 1997; 139
N.A. Stover (pgen.1010194.ref102) 2006; 34
J.S. Briguglio (pgen.1010194.ref035) 2013; 203
F. Skouri (pgen.1010194.ref042) 1997; 8
J. Zivanov (pgen.1010194.ref070) 2018; 7
J. Frankel (pgen.1010194.ref067) 2000; 62
References_xml – volume: 29
  start-page: 1823
  year: 2012
  ident: pgen.1010194.ref066
  article-title: Origin and evolution of laminin gene family diversity
  publication-title: Mol Biol Evol
  doi: 10.1093/molbev/mss060
– volume: 6
  start-page: 19
  year: 2006
  ident: pgen.1010194.ref077
  article-title: Secretion of functional human enzymes by Tetrahymena thermophila
  publication-title: BMC Biotechnol
  doi: 10.1186/1472-6750-6-19
– volume: 80
  start-page: 100807
  year: 2021
  ident: pgen.1010194.ref010
  article-title: Maturing secretory granules: Where secretory and endocytic pathways converge
  publication-title: Adv Biol Regul
  doi: 10.1016/j.jbior.2021.100807
– volume: 129
  start-page: 592
  year: 2016
  ident: pgen.1010194.ref014
  article-title: Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis
  publication-title: J Cell Sci
  doi: 10.1242/jcs.178285
– volume: 6
  start-page: 303
  year: 2005
  ident: pgen.1010194.ref049
  article-title: Core formation and the acquisition of fusion competence are linked during secretory granule maturation in Tetrahymena
  publication-title: Traffic
  doi: 10.1111/j.1600-0854.2005.00273.x
– volume: 206
  start-page: 873
  year: 2017
  ident: pgen.1010194.ref060
  article-title: The Hippo Pathway Maintains the Equatorial Division Plane in the Ciliate Tetrahymena
  publication-title: Genetics
  doi: 10.1534/genetics.117.200766
– volume: 7
  start-page: 56
  year: 2013
  ident: pgen.1010194.ref076
  article-title: Laminins in basement membrane assembly
  publication-title: Cell Adh Migr
  doi: 10.4161/cam.21831
– volume: 20
  start-page: 404
  year: 2019
  ident: pgen.1010194.ref011
  article-title: The road to lysosome-related organelles: Insights from Hermansky-Pudlak syndrome and other rare diseases
  publication-title: Traffic
  doi: 10.1111/tra.12646
– volume: 8
  start-page: 486
  year: 2007
  ident: pgen.1010194.ref015
  article-title: The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epithelium
  publication-title: Traffic
  doi: 10.1111/j.1600-0854.2007.00548.x
– volume: 111
  start-page: 131
  issue: Pt 1
  year: 1998
  ident: pgen.1010194.ref048
  article-title: Mutational analysis of regulated exocytosis in Tetrahymena
  publication-title: J Cell Sci
  doi: 10.1242/jcs.111.1.131
– volume: 2013
  year: 2013
  ident: pgen.1010194.ref065
  article-title: Tetrahymena functional genomics database (TetraFGD): an integrated resource for Tetrahymena functional genomics
  publication-title: Database: the journal of biological databases and curation
  doi: 10.1093/database/bat008
– volume: 16
  start-page: 4046
  year: 2005
  ident: pgen.1010194.ref052
  article-title: Genetic, genomic, and functional analysis of the granule lattice proteins in Tetrahymena secretory granules
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e05-01-0028
– volume: 115
  start-page: 453
  year: 2021
  ident: pgen.1010194.ref022
  article-title: Biogenesis and discharge of the rhoptries: Key organelles for entry and hijack of host cells by the Apicomplexa
  publication-title: Mol Microbiol
  doi: 10.1111/mmi.14674
– year: 2021
  ident: pgen.1010194.ref044
  article-title: An Alveolata secretory machinery adapted to parasite host cell invasion
  publication-title: Nat Microbiol
– volume: 18
  start-page: 18
  year: 2017
  ident: pgen.1010194.ref051
  article-title: An evolutionary balance: conservation vs innovation in ciliate membrane trafficking
  publication-title: Traffic
  doi: 10.1111/tra.12450
– volume: 90
  start-page: 844
  year: 2011
  ident: pgen.1010194.ref083
  article-title: RNAi knockdown of parafusin inhibits the secretory pathway
  publication-title: Eur J Cell Biol
  doi: 10.1016/j.ejcb.2011.06.002
– volume: 39
  start-page: 136
  year: 2015
  ident: pgen.1010194.ref084
  article-title: Evolutionary implications of localization of the signaling scaffold protein parafusin to both cilia and the nucleus
  publication-title: Cell Biol Int
  doi: 10.1002/cbin.10337
– volume: 5
  year: 2016
  ident: pgen.1010194.ref094
  article-title: Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
  publication-title: Elife
  doi: 10.7554/eLife.19090
– volume: 10
  start-page: 4041
  year: 2019
  ident: pgen.1010194.ref046
  article-title: A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites
  publication-title: Nat Commun
  doi: 10.1038/s41467-019-11979-z
– volume: 83
  start-page: 581
  year: 2003
  ident: pgen.1010194.ref009
  article-title: Secretory granule exocytosis
  publication-title: Physiol Rev
  doi: 10.1152/physrev.00031.2002
– volume: 139
  start-page: 1197
  year: 1997
  ident: pgen.1010194.ref086
  article-title: In vivo analysis of the major exocytosis-sensitive phosphoprotein in Tetrahymena
  publication-title: J. Cell Biol
  doi: 10.1083/jcb.139.5.1197
– volume: 280
  start-page: 79
  year: 2010
  ident: pgen.1010194.ref082
  article-title: Membrane trafficking in protozoa SNARE proteins, H+-ATPase, actin, and other key players in ciliates
  publication-title: Int Rev Cell Mol Biol
– volume: 11
  start-page: 515
  year: 2012
  ident: pgen.1010194.ref036
  article-title: Toxoplasma Sortilin-like Receptor Regulates Protein Transport and Is Essential for Apical Secretory Organelle Biogenesis and Host Infection
  publication-title: Cell Host Microbe
  doi: 10.1016/j.chom.2012.03.006
– volume: 3
  start-page: e1602552
  year: 2017
  ident: pgen.1010194.ref055
  article-title: Microbial arms race: Ballistic "nematocysts" in dinoflagellates represent a new extreme in organelle complexity
  publication-title: Sci Adv
  doi: 10.1126/sciadv.1602552
– volume: 68
  start-page: 419
  year: 1995
  ident: pgen.1010194.ref085
  article-title: Mammalian homologue of the calcium-sensitive phosphoglycoprotein, parafusin
  publication-title: Eur. J. Cell Biol
– volume: 82
  start-page: 269
  year: 2000
  ident: pgen.1010194.ref054
  article-title: Molecular genetics of regulated secretion in Paramecium
  publication-title: Biochimie
  doi: 10.1016/S0300-9084(00)00201-7
– volume: 34
  start-page: D500
  year: 2006
  ident: pgen.1010194.ref102
  article-title: Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila research
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkj054
– volume: 118
  year: 2021
  ident: pgen.1010194.ref019
  article-title: Single-cell visualization and quantification of trace metals in Chlamydomonas lysosome-related organelles
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.2026811118
– volume: 50
  start-page: 383
  year: 2003
  ident: pgen.1010194.ref030
  article-title: Extrusomes in ciliates: diversification, distribution, and phylogenetic implications
  publication-title: J Eukaryot Microbiol
  doi: 10.1111/j.1550-7408.2003.tb00260.x
– volume: 4
  start-page: 2129
  year: 2005
  ident: pgen.1010194.ref041
  article-title: Nd6p, a novel protein with RCC1-like domains involved in exocytosis in Paramecium tetraurelia
  publication-title: Eukaryot Cell
  doi: 10.1128/EC.4.12.2129-2139.2005
– volume: 146
  start-page: 135
  year: 1997
  ident: pgen.1010194.ref097
  article-title: Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment
  publication-title: Genetics
  doi: 10.1093/genetics/146.1.135
– volume: 5
  start-page: 63
  year: 2004
  ident: pgen.1010194.ref050
  article-title: Out with a bang! Tetrahymena as a model system to study secretory granule biogenesis
  publication-title: Traffic
  doi: 10.1046/j.1600-0854.2003.00155.x
– volume: 59
  start-page: 147
  year: 2019
  ident: pgen.1010194.ref005
  article-title: Lysosome-related organelles as functional adaptations of the endolysosomal system
  publication-title: Curr Opin Cell Biol
  doi: 10.1016/j.ceb.2019.05.003
– volume: 490
  start-page: 201
  year: 2012
  ident: pgen.1010194.ref071
  article-title: Molecular machines governing exocytosis of synaptic vesicles
  publication-title: Nature
  doi: 10.1038/nature11320
– volume: 82
  start-page: 342
  year: 2010
  ident: pgen.1010194.ref096
  article-title: Modules for C-terminal epitope tagging of Tetrahymena genes
  publication-title: J Microbiol Methods
  doi: 10.1016/j.mimet.2010.07.009
– volume: 17
  start-page: 250
  year: 1970
  ident: pgen.1010194.ref032
  article-title: Capture and ingestion of Paramecium by Didinium nasutum
  publication-title: J Protozool
  doi: 10.1111/j.1550-7408.1970.tb02366.x
– volume: 64
  start-page: 49
  year: 1983
  ident: pgen.1010194.ref047
  article-title: Isolation and ultrastructural characterization of secretory mutants of Tetrahymena thermophila
  publication-title: J. Cell Sci
  doi: 10.1242/jcs.64.1.49
– volume: 4
  start-page: 2466
  year: 2019
  ident: pgen.1010194.ref027
  article-title: Vibrio cholerae residing in food vacuoles expelled by protozoa are more infectious in vivo
  publication-title: Nat Microbiol
  doi: 10.1038/s41564-019-0563-x
– volume: 260
  start-page: 84
  year: 1991
  ident: pgen.1010194.ref033
  article-title: Defensive function of trichocysts in Paramecium
  publication-title: J. Exp. Zool
  doi: 10.1002/jez.1402600111
– volume: 6
  start-page: 1313
  year: 2015
  ident: pgen.1010194.ref029
  article-title: The Role of Ciliate Protozoa in the Rumen
  publication-title: Front Microbiol
  doi: 10.3389/fmicb.2015.01313
– volume: 8
  start-page: 1498
  year: 2016
  ident: pgen.1010194.ref078
  article-title: Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activity
  publication-title: MAbs
  doi: 10.1080/19420862.2016.1228504
– volume: 3
  start-page: a009712
  year: 2011
  ident: pgen.1010194.ref073
  article-title: The thrombospondins
  publication-title: Cold Spring Harb Perspect Biol
  doi: 10.1101/cshperspect.a009712
– volume: 1641
  start-page: 183
  year: 2003
  ident: pgen.1010194.ref056
  article-title: Dense-core secretory vesicle docking and exocytotic membrane fusion in Paramecium cells
  publication-title: Biochim Biophys Acta
  doi: 10.1016/S0167-4889(03)00092-2
– volume: 180
  start-page: 195
  year: 1989
  ident: pgen.1010194.ref068
  article-title: Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases
  publication-title: Anal Biochem
  doi: 10.1016/0003-2697(89)90115-2
– volume: 157
  start-page: 611
  year: 2001
  ident: pgen.1010194.ref039
  article-title: ND9P, a novel protein with armadillo-like repeats involved in exocytosis: physiological studies using allelic mutants in paramecium
  publication-title: Genetics
  doi: 10.1093/genetics/157.2.611
– year: 2014
  ident: pgen.1010194.ref061
  article-title: An aspartyl cathepsin, CTH3, is essential for proprotein processing during secretory granule maturation in Tetrahymena thermophila
  publication-title: Molecular biology of the cell
– volume: 28
  start-page: 1551
  year: 2017
  ident: pgen.1010194.ref063
  article-title: An endosomal syntaxin and the AP-3 complex are required for formation and maturation of candidate lysosome-related secretory organelles (mucocysts) in Tetrahymena thermophila
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e17-01-0018
– volume: 133
  year: 2020
  ident: pgen.1010194.ref099
  article-title: Diversification of CORVET tethers facilitates transport complexity in Tetrahymena thermophila
  publication-title: J Cell Sci
  doi: 10.1242/jcs.238659
– volume: 9
  start-page: 497
  year: 1998
  ident: pgen.1010194.ref079
  article-title: Proteolytic processing and Ca2+-binding activity of dense-core vesicle polypeptides in Tetrahymena
  publication-title: Mol. Biol. Cell
  doi: 10.1091/mbc.9.2.497
– volume: 25
  start-page: 495
  year: 2013
  ident: pgen.1010194.ref003
  article-title: Lysosome-related organelles: unusual compartments become mainstream
  publication-title: Current opinion in cell biology
  doi: 10.1016/j.ceb.2013.04.008
– volume: 52
  start-page: 291
  year: 2005
  ident: pgen.1010194.ref053
  article-title: Genomic and proteomic evidence for a second family of dense core granule cargo proteins in Tetrahymena thermophila
  publication-title: J Eukaryot Microbiol
  doi: 10.1111/j.1550-7408.2005.00045.x
– volume: 13
  start-page: 2513
  year: 2014
  ident: pgen.1010194.ref100
  article-title: Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ
  publication-title: Mol Cell Proteomics
  doi: 10.1074/mcp.M113.031591
– volume: 28
  start-page: 697
  year: 2018
  ident: pgen.1010194.ref038
  article-title: Remodeling the Specificity of an Endosomal CORVET Tether Underlies Formation of Regulated Secretory Vesicles in the Ciliate Tetrahymena thermophila
  publication-title: Curr Biol
  doi: 10.1016/j.cub.2018.01.047
– volume: 21
  start-page: e13018
  year: 2019
  ident: pgen.1010194.ref023
  article-title: Biogenesis and secretion of micronemes in Toxoplasma gondii
  publication-title: Cell Microbiol
  doi: 10.1111/cmi.13018
– volume: 56
  start-page: 56
  year: 2019
  ident: pgen.1010194.ref075
  article-title: Laminin G-like domains: dystroglycan-specific lectins
  publication-title: Curr Opin Struct Biol
  doi: 10.1016/j.sbi.2018.11.007
– volume: 26
  start-page: 1367
  year: 2008
  ident: pgen.1010194.ref101
  article-title: MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.1511
– volume: 135
  start-page: 1775
  year: 1996
  ident: pgen.1010194.ref059
  article-title: Granule lattice protein 1 (Grl1p), an acidic, calcium-binding protein in Tetrahymena thermophila dense-core secretory granules, influences granule size, shape, content organization, and release but not protein sorting or condensation
  publication-title: J Cell Biol
  doi: 10.1083/jcb.135.6.1775
– volume: 23
  start-page: 4444
  year: 2012
  ident: pgen.1010194.ref013
  article-title: MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway
  publication-title: Mol Biol Cell
  doi: 10.1091/mbc.e12-05-0369
– volume: 6
  start-page: 33555
  year: 2016
  ident: pgen.1010194.ref025
  article-title: The Tara Oceans voyage reveals global diversity and distribution patterns of marine planktonic ciliates
  publication-title: Sci Rep
  doi: 10.1038/srep33555
– volume: 9
  start-page: 357
  year: 2012
  ident: pgen.1010194.ref089
  article-title: Fast gapped-read alignment with Bowtie 2
  publication-title: Nat Methods
  doi: 10.1038/nmeth.1923
– year: 2009
  ident: pgen.1010194.ref057
  article-title: Independent transport and sorting of functionally distinct protein families in Tetrahymena dense core secretory granules
  publication-title: Eukaryot Cell
– volume: 293
  start-page: 345
  year: 2018
  ident: pgen.1010194.ref018
  article-title: Munc13 proteins control regulated exocytosis in mast cells
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M117.816884
– volume: 62
  start-page: 253
  year: 2000
  ident: pgen.1010194.ref087
  article-title: Genetically sorting a collection of Tetrahymena mutants
  publication-title: Methods Cell Biol
  doi: 10.1016/S0091-679X(08)61535-6
– volume: 308
  start-page: 751
  year: 2018
  ident: pgen.1010194.ref024
  article-title: Secretory organelle trafficking in Toxoplasma gondii: A long story for a short travel
  publication-title: Int J Med Microbiol
  doi: 10.1016/j.ijmm.2018.07.007
– volume: 201
  start-page: 3051
  year: 2018
  ident: pgen.1010194.ref045
  article-title: Myoferlin-Mediated Lysosomal Exocytosis Regulates Cytotoxicity by Phagocytes
  publication-title: J Immunol
  doi: 10.4049/jimmunol.1800268
– volume: 10
  start-page: 1979
  year: 1991
  ident: pgen.1010194.ref072
  article-title: Maturation of dense core granules in wild type and mutant Tetrahymena thermophila
  publication-title: EMBO J
  doi: 10.1002/j.1460-2075.1991.tb07727.x
– volume: 19
  start-page: 394
  year: 2007
  ident: pgen.1010194.ref004
  article-title: Lysosome-related organelles: driving post-Golgi compartments into specialisation
  publication-title: Current opinion in cell biology
  doi: 10.1016/j.ceb.2007.05.001
– volume: 8
  start-page: 1063
  year: 1997
  ident: pgen.1010194.ref042
  article-title: Genetic approach to regulated exocytosis using functional complementation in Paramecium: identification of the ND7 gene required for membrane fusion
  publication-title: Mol. Biol. Cell
  doi: 10.1091/mbc.8.6.1063
– volume: 61
  start-page: 331
  year: 2017
  ident: pgen.1010194.ref026
  article-title: Functional diversity of aquatic ciliates
  publication-title: Eur J Protistol
  doi: 10.1016/j.ejop.2017.04.001
– volume: 69
  start-page: 126
  year: 1976
  ident: pgen.1010194.ref043
  article-title: Genetic analysis of membrane differentiation in Paramecium: freeze-fracture study of the trichocyst cycle in wild-type and mutant strains
  publication-title: J. Cell Biol
  doi: 10.1083/jcb.69.1.126
– volume: 7
  start-page: 195
  year: 2019
  ident: pgen.1010194.ref006
  article-title: Mechanism of Acrosome Biogenesis in Mammals
  publication-title: Front Cell Dev Biol
  doi: 10.3389/fcell.2019.00195
– volume: 13
  start-page: 731
  year: 2016
  ident: pgen.1010194.ref103
  article-title: The Perseus computational platform for comprehensive analysis of (prote)omics data
  publication-title: Nat Methods
  doi: 10.1038/nmeth.3901
– volume: 10
  start-page: 623
  year: 2009
  ident: pgen.1010194.ref002
  article-title: Lysosome biogenesis and lysosomal membrane proteins: trafficking meets function
  publication-title: Nat Rev Mol Cell Biol
  doi: 10.1038/nrm2745
– volume: 203
  start-page: 537
  year: 2013
  ident: pgen.1010194.ref035
  article-title: Lysosomal sorting receptors are essential for secretory granule biogenesis in Tetrahymena
  publication-title: The Journal of cell biology
  doi: 10.1083/jcb.201305086
– volume: 143
  start-page: 1
  year: 2016
  ident: pgen.1010194.ref021
  article-title: Diversity of extracellular proteins during the transition from the ’proto-apicomplexan’ alveolates to the apicomplexan obligate parasites
  publication-title: Parasitology
  doi: 10.1017/S0031182015001213
– volume: 6
  start-page: 80
  year: 2012
  ident: pgen.1010194.ref093
  article-title: A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3
  publication-title: Fly (Austin)
  doi: 10.4161/fly.19695
– volume: 6
  year: 2017
  ident: pgen.1010194.ref016
  article-title: Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa
  publication-title: Elife
– volume: 62
  start-page: 27
  year: 2000
  ident: pgen.1010194.ref067
  article-title: Cell biology of Tetrahymena thermophila
  publication-title: Methods Cell Biol
  doi: 10.1016/S0091-679X(08)61528-9
– volume: 8
  start-page: 523
  year: 2007
  ident: pgen.1010194.ref081
  article-title: Molecular identification of 26 syntaxin genes and their assignment to the different trafficking pathways in Paramecium
  publication-title: Traffic
  doi: 10.1111/j.1600-0854.2007.00544.x
– volume: 61
  start-page: 293
  year: 2014
  ident: pgen.1010194.ref031
  article-title: Chemical offense by means of toxicysts in the freshwater ciliate, Coleps hirtus
  publication-title: The Journal of eukaryotic microbiology
  doi: 10.1111/jeu.12106
– volume: 8
  start-page: 204
  year: 1992
  ident: pgen.1010194.ref034
  article-title: Invasion and development strategies of Ichthyophthirius multifiliis, a parasitic ciliate of fish
  publication-title: Parasitol Today
  doi: 10.1016/0169-4758(92)90265-4
– volume: 6
  start-page: a016840
  year: 2014
  ident: pgen.1010194.ref001
  article-title: The biogenesis of lysosomes and lysosome-related organelles
  publication-title: Cold Spring Harb Perspect Biol
  doi: 10.1101/cshperspect.a016840
– volume: 14
  start-page: 817
  year: 2015
  ident: pgen.1010194.ref062
  article-title: Secretion of Polypeptide Crystals from Tetrahymena thermophila Secretory Organelles (Mucocysts) Depends on Processing by a Cysteine Cathepsin, Cth4p
  publication-title: Eukaryotic cell
  doi: 10.1128/EC.00058-15
– volume: 55
  start-page: 574
  year: 2020
  ident: pgen.1010194.ref074
  article-title: NLR-1/CASPR Anchors F-Actin to Promote Gap Junction Formation
  publication-title: Dev Cell
  doi: 10.1016/j.devcel.2020.10.020
– volume: 4
  start-page: e4429
  year: 2009
  ident: pgen.1010194.ref064
  article-title: Microarray analyses of gene expression during the Tetrahymena thermophila life cycle
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0004429
– volume: 159
  start-page: 1605
  year: 2001
  ident: pgen.1010194.ref058
  article-title: Analysis of a mutant exhibiting conditional sorting to dense core secretory granules in Tetrahymena thermophila
  publication-title: Genetics
  doi: 10.1093/genetics/159.4.1605
– volume: 7
  year: 2018
  ident: pgen.1010194.ref070
  article-title: New tools for automated high-resolution cryo-EM structure determination in RELION-3
  publication-title: Elife
  doi: 10.7554/eLife.42166
– volume: 2
  year: 2017
  ident: pgen.1010194.ref069
  article-title: A Glycosylphosphatidylinositol-Anchored Carbonic Anhydrase-Related Protein of Toxoplasma gondii Is Important for Rhoptry Biogenesis and Virulence
  publication-title: mSphere
  doi: 10.1128/mSphere.00027-17
– volume: 211
  start-page: 651
  year: 2019
  ident: pgen.1010194.ref090
  article-title: Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the Ciliate Tetrahymena
  publication-title: Genetics
  doi: 10.1534/genetics.118.301889
– volume: 1868
  start-page: 118947
  year: 2021
  ident: pgen.1010194.ref020
  article-title: The IP3 receptor and Ca(2+) signaling in trypanosomes
  publication-title: Biochim Biophys Acta Mol Cell Res
  doi: 10.1016/j.bbamcr.2021.118947
– volume: 17
  start-page: 1157
  year: 2015
  ident: pgen.1010194.ref037
  article-title: Toxoplasma gondii Vps11, a subunit of HOPS and CORVET tethering complexes, is essential for the biogenesis of secretory organelles
  publication-title: Cell Microbiol
  doi: 10.1111/cmi.12426
– volume: 169
  start-page: 321
  year: 2018
  ident: pgen.1010194.ref105
  article-title: Grl1 Protein is a Candidate K Antigen in Tetrahymena thermophila
  publication-title: Protist
  doi: 10.1016/j.protis.2018.03.004
– volume: 62
  start-page: 189
  year: 2000
  ident: pgen.1010194.ref088
  article-title: Tetrahymena as a laboratory organism: useful strains, cell culture, and cell line maintenance
  publication-title: Methods Cell Biol
  doi: 10.1016/S0091-679X(08)61530-7
– volume: 201
  start-page: 700
  year: 2018
  ident: pgen.1010194.ref017
  article-title: C2 Domains of Munc13-4 Are Crucial for Ca(2+)-Dependent Degranulation and Cytotoxicity in NK Cells
  publication-title: J Immunol
  doi: 10.4049/jimmunol.1800426
– volume: 5
  start-page: 493
  year: 2004
  ident: pgen.1010194.ref040
  article-title: Novel secretory vesicle proteins essential for membrane fusion display extracellular-matrix domains
  publication-title: Traffic
  doi: 10.1111/j.1600-0854.2004.00194.x
– volume: 12
  start-page: R100
  year: 2011
  ident: pgen.1010194.ref028
  article-title: Comparative genomics of the pathogenic ciliate Ichthyophthirius multifiliis, its free-living relatives and a host species provide insights into adoption of a parasitic lifestyle and prospects for disease control
  publication-title: Genome Biol
  doi: 10.1186/gb-2011-12-10-r100
– volume: 130
  start-page: 3611
  year: 2017
  ident: pgen.1010194.ref008
  article-title: Weibel-Palade bodies at a glance
  publication-title: J Cell Sci
  doi: 10.1242/jcs.208033
– volume: 33
  start-page: 652
  year: 2005
  ident: pgen.1010194.ref012
  article-title: Targeting of Rab GTPases to cellular membranes
  publication-title: Biochem Soc Trans
  doi: 10.1042/BST0330652
– year: 2016
  ident: pgen.1010194.ref080
  article-title: Whole Genome Sequencing Identifies a Novel Factor Required for Secretory Granule Maturation in Tetrahymena thermophila
  publication-title: G3
– volume: 4
  start-page: e286
  year: 2006
  ident: pgen.1010194.ref092
  article-title: Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote
  publication-title: PLoS biology
  doi: 10.1371/journal.pbio.0040286
– volume: 425
  start-page: 79
  year: 2008
  ident: pgen.1010194.ref095
  article-title: High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene
  publication-title: Gene
  doi: 10.1016/j.gene.2008.08.007
– volume: 47
  start-page: D442
  year: 2019
  ident: pgen.1010194.ref104
  article-title: The PRIDE database and related tools and resources in 2019: improving support for quantification data
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gky1106
– year: 2021
  ident: pgen.1010194.ref007
  article-title: Melanosome biogenesis in the pigmentation of mammalian skin
  publication-title: Integr Comp Biol
– volume: 46
  start-page: W537
  year: 2018
  ident: pgen.1010194.ref091
  article-title: The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gky379
– volume: 1411
  start-page: 67
  year: 2016
  ident: pgen.1010194.ref098
  article-title: High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes
  publication-title: Methods Mol Biol
  doi: 10.1007/978-1-4939-3530-7_3
SSID ssj0035897
Score 2.416319
Snippet In the ciliate Tetrahymena thermophila , lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in...
In the ciliate Tetrahymena thermophila, lysosome-related organelles called mucocysts accumulate at the cell periphery where they secrete their contents in...
SourceID plos
doaj
pubmedcentral
proquest
gale
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage e1010194
SubjectTerms Animals
Biology and Life Sciences
Cell membranes
Ciliates
Defects
Exocytosis
Exocytosis - genetics
Genetic aspects
Localization
Lysosomes
Lysosomes - metabolism
Macromolecules
Mutation
Organelles
Organelles - metabolism
Physiological aspects
Plasma
Proteins
Research and Analysis Methods
Secretory Vesicles - genetics
Secretory Vesicles - metabolism
Signal transduction
Tetrahymena
Tetrahymena thermophila
Tetrahymena thermophila - genetics
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELdQJSReEN8rDDAIiaewNnbs5rEgpoHEkGBDe4uc-LxWau1qadH6zD_OnZNGC5q0PfDa-yVt7y730Z5_x9g7PapSUEInLkczSJCQTMaqTCZVXjphK7CRge_bsTo6lV_PsrMrq75oJqyhB24Ud6Ak5khAt3GYuVMJpRSVHIlUY5ARYxejL-a8XTPVxGCRTZq1KlkmEo1tfXtoTujxQWujDys0EPWuWOPIXlKK3P1dhB6sFqG-rvz8d4rySlo6fMDut_Uknzbf4yG7A_4Ru9tsmNw-Zn-m3IffsOBLWGJf7IHHGXK45POaXwCNAYPlWLhyi4ER0xg33qIgLqhHCVyGarsONaKD44ttHeqwhCSegEFx3AlFP_3XfO75CeAnnG2X4A2nunIZVrP5wjxhp4efTz4dJe3ehaTSqVonuR05IUsDo9QKbJ9lZUtj3QSM1qAnVlmNZZg1aMjcGINFQAkZqkHmNnNOlOIpG_jgYY9xIm92mdSpcQ4NibmwrCDDt8kF6Az0kImd4ouqJSWn3RiLIv7TprE5afRYkLmK1lxDlnRXrRpSjhvwH8mmHZYoteMLqKWidbTiJkcbstfkEUVzPrULDMVUY8mraXX8kL2NCKLV8DS3c242dV18-f7rFqCfx7cB_eiB3rcgF1BnlWkPVKDmidOrh9zvITGCVD3xHjn5TnV1kSqNaQeb1Qyv3Dn-9eI3nZhuSgN7HsKGMErhrVWOenvWPCed-onRX8tcDZnuPUE9-_Qlfj6L1Oc5Vt_41s__h0FfsHspnWUhKt58nw3WFxt4iRXmunwVg8lf2516WQ
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Scholars Portal Journals: Open Access
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELdGERIviO8VBhiExFOmNnbs5AGhgpgG0oYEK9pb5MRnWqlNStOi9pl_nDsnjQgqsNfcz451Z9-HfL5j7KUe5CEooQOXoBgkSAjiocqCOE8yJ2wO1lfgOztXp2P58TK6PGC7nq0NA6u9oR31kxovZ8eb79s3eOBf-64NergbdLxAllM0il6LvMauo23S1NPgTLb3CiKK63YrUSQCjeF-85jub7N0jJWv6d9q7t5iVlb73NI_syt_M1cnt9mtxs_ko3pj3GEHUNxlN-rOk9t77OeIF-UPmPE5zDFeLoD73HLY8GnFl0DpwWA5OrTcosJE88ZNYZHgG9cjBTZlvl2VFaJLx2fbqqzKOQT-ZQySfa8ouhKo-LTgF4ArnGznUBhO_ua8XEymM3OfjU_eX7w7DZp-DEGuQ7UKEjtwQmYGBqEVGFbL3GbGuhiM1qBjq6xG98waFHBijEHnIIMI2SATGzknMvGA9YqygEPGqaizi6QOjXNSRWgjsxwi_E0iQEeg-0zsGJ_mTbFy6pkxS_0NnMagpeZjSuJKG3H1WdCOWtTFOv6Df0sybbFUatt_QC6lzclNFa4vBtRbDl3HUEImRS4HItRo5cTQhX32jHZEWr9bbRVGOtLoCmtqKd9nLzyCym0UlM_zzayrKv3w6esVQF_OrwL63AG9akCuRJ7lpnlogZynWl8d5FEHiZol75APaZPvWFelodJojjCIjXDkbuPvJz9vyTQpJfIVUK4JoxROrRLk28P6nLTsp0r_Wiaqz3TnBHXk06UU04kviZ6gV46_fvTvZT1mN0N6vULFd5Mj1lst1_AEfcpV9tSriV9SmHkI
  priority: 102
  providerName: Scholars Portal
Title A novel membrane complex is required for docking and regulated exocytosis of lysosome-related organelles in Tetrahymena thermophila
URI https://www.ncbi.nlm.nih.gov/pubmed/35587496
https://www.proquest.com/docview/2677632175
https://www.proquest.com/docview/2666908692
https://pubmed.ncbi.nlm.nih.gov/PMC9159632
https://doaj.org/article/64658e179f64424eb43c40327eb131f2
http://dx.doi.org/10.1371/journal.pgen.1010194
Volume 18
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3db9MwELegExIviO8VRjEIiaewNnbi5Al1aNNAWkFjQ3uLnPi8VmrjsnRofeYf585JA0ET7KUPvUti3dnn3_njd4y9UcMihFiowKboBgkSgmQU50FSpLkVpgDjGfiOJvHhqfx0Fp01C25Vc6xyExN9oDauoDXy3TBWOBQQQEfvl98DqhpFu6tNCY3bbIuoy6hXq7M24RJRUhdXiSIRKEzum6tzQo12G0-9W6KbKINFpCM7U5Nn8G_jdG85d9V1IPTvs5R_TE4H99m9BlXycd0NHrBbUD5kd-o6k-tH7OeYl-4HzPkCFpgdl8D9SXK44rOKXwAdBgbDEb5ytAOtnXNdGhT4MvUogStXrFeuQm1n-XxducotIPD3YFDsK0PRBkDFZyU_AWzhdL2AUnNClwu3nM7m-jE7Pdg_-XAYNNUXgkKF8SpIzdAKmWsYhkZgEi0Lk2tjE9BKgUpMbBSCMaPRnanWGqFADhGaQaYmslbk4gnrla6EbcaJwtlGUoXaWhlHOCPmBUT4mVSAikD1mdgYPisaanKqkDHP_H6bwhSltmNG7soad_VZ0D61rKk5_qO_Rz5tdYlY2_-BVsqacZrF2L4EMEpZBIqhhFyKQg5FqHBOEyMb9tlL6hFZfUu1DQ_ZWCHwVVRAvs9eew0i1yjp9M65vqyq7OPnbzdQ-jq5idJxR-lto2Qd2qzQzbUKtDwxe3U0dzqaGEeKjnibOvnGdFX2e8Thk5uOf734VSuml9KxvRLcJenEMb46TtFuT-tx0pqfeP2VTOM-U50R1PFPV1LOpp4APUUMjp9-9u9mPWd3Q7qrQlS76Q7rrS4u4QUiyFU-8GFiwLb29idfjgd-HQZ_j2TyCze9drY
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR3LbtNAcFWCEFwQ7wYKXRCIk6njXXvjA0LhUSW0DRK0KDez9s42kRI71Ak0Z_6Hb2TGdgxGFfTSq2e8Xs3MzsM7D8aeKjfxIBDKsSGyQYIEp9sJYqebhLEVJgFTdOA7GAb9I_l-5I822M91LQylVa51YqGoTZbQP_IdL1B4FNCB9l_Nvzo0NYpuV9cjNEqx2IPVdwzZ8peDt8jfZ563--7wTd-ppgo4ifKChRMa1woZa3A9IzA4lImJtbFd0EqB6prAKHQyjMZthlprNHEx-LhpGRrfWhELXPcSu4yG16VgT43qAE_43XKYi-8LR4nQrUr1hOrsVJLxYo5iQREzelayYQqLiQG1XWjNp1l-ltP7d-7mH8Zw9wa7XnmxvFeK3U22AektdqWca7m6zX70eJp9gymfwQyj8RR4kbkOp3yS8xOg5GMwHN1ljnSnf_VcpwYBxzRLDCFwmiWrRZYjdmb5dJVneTYDp6i7QXAxiYouHHI-Sfkh4A7HqxmkmpM3O8vm48lU32FHF8KXu6yVZilsMk4to60vlaetlYGPFjhOwMfPhAKUD6rNxJrwUVK1QqeJHNOouN9TGBKVdIyIXVHFrjZz6rfmZSuQ_-C_Jp7WuNTIu3iAVIoqvRAFuL8uoFa06Jh6EmIpEukKT6ENFR3rtdk2SURUVsXW6ijqKXS0FQ2sb7MnBQY180gpW-hYL_M8Gnz4fA6kT8PzIH1sID2vkGyGNEt0VcaBlKdOYg3MrQYm6q2kAd4kIV-TLo9-n3B8cy34Z4Mf12BalNIEU8iWhBMEuHQQIt3uleekJj_NEVAyDNpMNU5Qgz9NSDoZFw3XQ_T58dP3_72tbXa1f3iwH-0PhnsP2DWP6mSozW-4xVqLkyU8RO91ET8qVAZnXy5aR_0Cnq2zuA
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtR3LbtNAcFVSgbgg3g0UuiAQJ5PEu_bGB4RS2qilEKrSVr2ZtXe2iZTYoU6gOfNXfB0zfgSMKuil18x4vZmZnYd3Hoy9UO3YBV8oxwbIBgkSnG7Hj5xuHERWmBhM3oHv48DfOZLvT7yTFfazqoWhtMpKJ-aK2qQxfSNvub7Co4AOtNeyZVrE_lb_7fSrQxOk6Ka1GqdRiMgeLL5j-Ja92d1CXr903f724bsdp5ww4MTK9WdOYNpWyEhD2zUCA0UZm0gb2wWtFKiu8Y1Ch8No3HKgtUZzF4GHf0AGxrNWRALXvcZWFUVFDba6uT3YP6jsgPC6xWgXzxOOEkG7LNwTqtMq5eT1FIWE4mf0s2TNMObzA5ZWojEdp9lFLvDfmZx_mMb-bXar9Gl5rxDCO2wFkrvsejHlcnGP_ejxJP0GYz6BCcbmCfA8jx3O-SjjZ0CpyGA4Os8cuUBf7rlODAJOabIYQuA8jRezNEPs1PLxIkuzdAJOXoWD4HwuFV0_ZHyU8EPAHQ4XE0g0J992kk6Ho7G-z46uhDMPWCNJE1hjnBpIW08qV1srfQ_tcRSDh68JBCgPVJOJivBhXDZGp_kc4zC_7VMYIBV0DIldYcmuJnOWT02LxiD_wd8kni5xqa13_gNSKSy1ROjj_rqAOtKim-pKiKSIZVu4Ci2q6Fi3yTZIIsKiRnapnMKeQrdb0fj6JnueY1Brj4QOyameZ1m4--n4EkifB5dBOqghvSqRbIo0i3VZ1IGUp75iNcz1GiZqsbgGXiMhr0iXhb_POz5ZCf7F4GdLMC1KSYMJpHPC8X1c2g-Qbg-Lc7IkP00VUDLwm0zVTlCNP3VIMhrm7dcDjADw1Y_-va0NdgP1U_hhd7D3mN10qWiGev4G66wxO5vDE3RlZ9HTUmdw9uWq1dQvHeq5Uw
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+novel+membrane+complex+is+required+for+docking+and+regulated+exocytosis+of+lysosome-related+organelles+in+Tetrahymena+thermophila&rft.jtitle=PLoS+genetics&rft.date=2022-05-19&rft.pub=Public+Library+of+Science&rft.issn=1553-7390&rft.eissn=1553-7404&rft.volume=18&rft.issue=5&rft.spage=e1010194&rft_id=info:doi/10.1371%2Fjournal.pgen.1010194&rft.externalDBID=HAS_PDF_LINK
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1553-7404&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1553-7404&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1553-7404&client=summon