Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males

• Published in: PLoS genetics Vol. 16; no. 11; p. e1009180
• Main Authors: Das, Siba R., Maselko, Maciej, Upadhyay, Ambuj, Smanski, Michael J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.11.2020; Public Library of Science (PLoS)
• Subjects: Animal genetic engineering; Animals; Animals, Genetically Modified - physiology; Antibiotics; Biology and Life Sciences; Chromosomes; Design; Drosophila melanogaster - genetics; Engineering and Technology; Female; Females; Gene expression; Gene pool; Genetic aspects; Genetic crosses; Genetic engineering; Genetic Engineering - methods; Genetic research; Insect Control - methods; Insect sterilization; Insects; Male; Males; Medicine and Health Sciences; Methods; Models, Animal; Pest Control, Biological - methods; Pests; Phenotypes; Research and Analysis Methods; Sex chromosomes; Sex Chromosomes - genetics; Sex Determination Analysis - methods; Sex Determination Processes - genetics; Sex preselection; Sex ratio; Statistical analysis; Transgenes - genetics; X chromosomes; Australia; United States

The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove on...