Meteorin-Like Shows Unique Expression Pattern in Bone and Its Overexpression Inhibits Osteoblast Differentiation

• Published in: PloS one Vol. 11; no. 10; p. e0164446
• Main Authors: Gong, Weiyan, Liu, Yong, Wu, Zhihong, Wang, Shaohai, Qiu, Guixing, Lin, Shouqing
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.10.2016; Public Library of Science (PLoS)
• Subjects: Activator protein 1; Adipokines - genetics; Analysis; Animals; Biocompatibility; Biology and Life Sciences; Bone and Bones - metabolism; Bone cancer; Cancellous bone; Cell differentiation; Cell Differentiation - genetics; Cell fusion; Cell Line; Cell Line, Tumor; CHO Cells; Chondrocytes; Chondrocytes - metabolism; Cloning; Cricetulus; Differentiation (biology); Fusion protein; Gene expression; Genes; Genetic research; Growth factors; Gynecology; HEK293 Cells; Humans; Immunohistochemistry; Intercellular Signaling Peptides and Proteins - genetics; Laboratory animals; Luciferase; Medicine and Health Sciences; Mineralization; Nerve Tissue Proteins - genetics; Obstetrics; Osteoblastogenesis; Osteoblasts; Osteoblasts - metabolism; Osteogenesis - genetics; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Proteins; Research and Analysis Methods; Rodents; Sarcoma; Transcription (Genetics); Transcription Factor AP-1 - metabolism; Transcription factors; Transcription, Genetic - genetics; Transfection; Transfection - methods

The present study was performed to identify and characterize genes involved in osteoblasts function. Firstly, we constructed and sequenced a human osteoblast full-length cDNA library to screen for genes whose functions have not been reported and further identify these candidate genes through detecti...