A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Most Relevant Members of the B. pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis

• Published in: PloS one Vol. 11; no. 10; p. e0164006
• Main Authors: Lowe, Chinn-Woan, Satterfield, Benjamin A, Nelson, Daniel B, Thiriot, Joseph D, Heder, Michael J, March, Jordon K, Drake, David S, Lew, Cynthia S, Bunnell, Annette J, Moore, Emily S, O'Neill, Kim L, Robison, Richard A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.10.2016; Public Library of Science (PLoS)
• Subjects: Assaying; Bacteria; Biology and Life Sciences; Burkholderia; Burkholderia - genetics; Burkholderia - isolation & purification; Burkholderia Infections - diagnosis; Burkholderia Infections - microbiology; Burkholderia mallei; Burkholderia mallei - genetics; Burkholderia mallei - isolation & purification; Burkholderia pseudomallei; Burkholderia pseudomallei - genetics; Burkholderia pseudomallei - isolation & purification; Differentiation; DNA, Bacterial - isolation & purification; Fatalities; Glanders; Glanders - microbiology; Humans; Identification methods; Infections; Infectious diseases; Laboratories; Medicine and Health Sciences; Melioidosis; Melioidosis - microbiology; Molecular biology; Oryza; Pathogens; Polymerase chain reaction; Real time; Real-Time Polymerase Chain Reaction - methods; Research and Analysis Methods; Sensitivity analysis; Sensitivity and Specificity; Sequence Analysis, DNA - methods; Species; Southeast Asia; Australia; Asia; United States > US

The Burkholderia pseudomallei complex classically consisted of B. mallei, B. pseudomallei, and B. thailandensis, but has now expanded to include B. oklahomensis, B. humptydooensis, and three unassigned Burkholderia clades. Methods for detecting and differentiating the B. pseudomallei complex has bee...