Artificial MicroRNAs as Novel Secreted Reporters for Cell Monitoring in Living Subjects

• Published in: PloS one Vol. 11; no. 7; p. e0159369
• Main Authors: Ronald, John A., D’Souza, Aloma L., Chuang, Hui-Yen, Gambhir, Sanjiv Sam
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.07.2016; Public Library of Science (PLoS)
• Subjects: Animals; Antigens; Bioindicators; Biology and life sciences; Bioluminescence; Biomarkers; Blood; Blood levels; Breast cancer; Cell culture; Cell number; Cell Tracking - methods; Cells; Cellular therapy; Culture media; Diagnostic systems; Genes; Genes, Reporter; Genetic aspects; HeLa Cells; Humans; Imaging; Imaging, Three-Dimensional; Implantation; In vitro methods and tests; Lung cancer; Media (culture); Medical imaging; Medical prognosis; Medicine and Health Sciences; Mice; MicroRNA; MicroRNAs; MicroRNAs - blood; MicroRNAs - metabolism; miRNA; Monitoring; mRNA; Multiplexing; NMR; Nuclear magnetic resonance; Patients; Pollution monitoring; Positron emission; Positron emission tomography; Proteins; Radiology; Reporter gene; Research and Analysis Methods; Ribonucleic acid; RNA; Secretion; Stem cells; Tomography; Transfection; Tumors; Ontario Canada; Canada; California; United States > US

Reporter genes are powerful technologies that can be used to directly inform on the fate of transplanted cells in living subjects. Imaging reporter genes are often employed to quantify cell number, location(s), and viability with various imaging modalities. To complement this, reporters that are sec...