Cell-Type Specific Responses to DNA Replication Stress in Early C. elegans Embryos

• Published in: PloS one Vol. 11; no. 10; p. e0164601
• Main Authors: Stevens, Holly, Williams, Ashley B., Michael, W. Matthew
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.10.2016; Public Library of Science (PLoS)
• Subjects: Animals; Ataxia Telangiectasia Mutated Proteins - antagonists & inhibitors; Ataxia Telangiectasia Mutated Proteins - genetics; Ataxia Telangiectasia Mutated Proteins - metabolism; Biology and Life Sciences; Blastomeres; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - antagonists & inhibitors; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cell cycle; Cellular stress response; Checkpoint Kinase 1 - antagonists & inhibitors; Checkpoint Kinase 1 - genetics; Checkpoint Kinase 1 - metabolism; Delay; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA damage; DNA Helicases - antagonists & inhibitors; DNA Helicases - genetics; DNA Helicases - metabolism; DNA replication; DNA Replication - drug effects; DNA Replication - radiation effects; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - metabolism; Embryo, Nonmammalian - radiation effects; Embryogenesis; Embryonic development; Embryonic Development - drug effects; Embryonic Development - radiation effects; Embryonic growth stage; Embryos; Hydroxyurea - toxicity; Interphase - drug effects; Interphase - radiation effects; Kinases; Nematodes; Physical sciences; Replication; Research and Analysis Methods; RNA Interference; RNA-mediated interference; Stress; Stress response; Stresses; Ultraviolet Rays; Worms; Los Angeles California; United States > US

To better understand how the cellular response to DNA replication stress is regulated during embryonic development, we and others have established the early C. elegans embryo as a model system to study this important problem. As is the case in most eukaryotic cell types, the replication stress respo...