Polysaccharide from black currant (Ribes nigrum L.) stimulates dendritic cells through TLR4 signaling

• Published in: Bioscience of Microbiota, Food and Health Vol. 36; no. 4; pp. 141 - 145
• Main Authors: ASHIGAI, Hiroshi, KOMANO, Yuta, WANG, Guanying, KAWACHI, Yasuji, SUNAGA, Kazuko, YAMAMOTO, Reiko, TAKATA, Ryoji, MIYAKE, Mika, YANAI, Takaaki
• Format: Journal Article
• Language: English; Japanese
• Published: Japan BMFH Press 2017; Japan Science and Technology Agency
• Subjects: Anticancer properties; Antitumor activity; black currant; dendritic cell; Dendritic cells; IL-1β; Immunology; immunostimulation; Interleukins; Lymphocytes T; MyD88 protein; polysaccharide; Ribes nigrum; Signaling; TLR4 protein; Toll-like receptors; Tumor necrosis factor-TNF; Tumor necrosis factor-α; black currant; polysaccharide; dendritic cell; immunostimulation
• ISSN: 2186-6953; 2186-3342; 2186-3342
• DOI: 10.12938/bmfh.16-029

Black currant (Ribes nigrum) has various beneficial properties for human health. In particular, polysaccharide from black currant was found to be an immunostimulating food ingredient and was reported to have antitumor activity in a mouse model. We named it cassis polysaccharide (CAPS). In a previous study, CAPS administration caused tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) production in vitro and in vivo, but the immunological mechanism of CAPS was not demonstrated. In this study, we revealed the CAPS immunostimulating mechanism in vitro. First, we found that CAPS activated dendritic cells (DCs). Second, we investigated whether it depends on Toll-like receptor 4 (TLR4) and myeloid differentiation primary response (Myd). We concluded that CAPS stimulates DCs through Myd88 depending TLR4 signaling and activates Th1-type cytokine release.