Bifidobacteria Abundance-Featured Gut Microbiota Compositional Change in Patients with Behcet's Disease

• Published in: PloS one Vol. 11; no. 4; p. e0153746
• Main Authors: Shimizu, Jun, Kubota, Takao, Takada, Erika, Takai, Kenji, Fujiwara, Naruyoshi, Arimitsu, Nagisa, Ueda, Yuji, Wakisaka, Sueshige, Suzuki, Tomoko, Suzuki, Noboru
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.04.2016; Public Library of Science (PLoS)
• Subjects: Abundance; Actinobacteria; Adolescent; Adult; Aged; Arthritis; Autoimmune diseases; Bacteria; Behcet Syndrome - microbiology; Behcet's syndrome; Bifidobacteria; Bifidobacterium; Bifidobacterium - isolation & purification; Biodiversity; Biology and Life Sciences; Complications and side effects; Computer and Information Sciences; Deoxyribonucleic acid; Discriminant analysis; Disease; DNA; Drug dosages; Dysbacteriosis; Fecal microflora; Feces; Female; Firmicutes; Gene sequencing; Genera; Genomes; Hospitals; Humans; Immunology; Intestinal microflora; Intestines - microbiology; Lactobacillaceae; Male; Medicine; Medicine and Health Sciences; Metabolism; Metabolites; Microbiota; Microbiota (Symbiotic organisms); Middle Aged; Patients; Physiological aspects; Prevotella; Relative abundance; Ribonucleic acid; RNA; rRNA 16S; Species diversity; Taxa; Vein & artery diseases; Young Adult; Japan
• DOI: 10.1371/journal.pone.0153746
• ISSN: 1932-6203

Gut microbiota compositional alteration may have an association with immune dysfunction in patients with Behcet's disease (BD). We conducted a fecal metagenomic analysis of BD patients. We analyzed fecal microbiota obtained from 12 patients with BD and 12 normal individuals by sequencing of 16S ribosomal RNA gene. We compared the relative abundance of bacterial taxa. Direct comparison of the relative abundance of bacterial taxa demonstrated that the genera Bifidobacterium and Eggerthella increased significantly and the genera Megamonas and Prevotella decreased significantly in BD patients compared with normal individuals. A linear discriminant analysis of bacterial taxa showed that the phylum Actinobacteria, including Bifidobacterium, and the family Lactobacillaceae exhibited larger positive effect sizes than other bacteria in patients with BD. The phylum Firmicutes and the class Clostridia had large effect sizes in normal individuals. There was no significant difference in annotated species numbers (as numbers of operational taxonomic unit; OTU) and bacterial diversity of each sample (alpha diversity) between BD patients and normal individuals. We next assigned each sample to a position using three axes by principal coordinates analysis of the OTU table. The two groups had a significant distance as beta diversity in the 3-axis space. Fecal sIgA concentrations increased significantly in BD patients but did not correlate with any bacterial taxonomic abundance. These data suggest that the compositional changes of gut microbes may be one type of dysbiosis (unfavorable microbiota alteration) in patients with BD. The dysbiosis may have an association with the pathophysiology of BD.