DNA Damage Responses Are Induced by tRNA Anticodon Nucleases and Hygromycin B

• Published in: PloS one Vol. 11; no. 7; p. e0157611
• Main Authors: Wemhoff, Sabrina, Klassen, Roland, Beetz, Anja, Meinhardt, Friedhelm
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.07.2016; Public Library of Science (PLoS)
• Subjects: Aminoglycosides; Analysis; Antibiotics; Apurinic sites; Baking yeast; Base excision repair; Biology and Life Sciences; Biosynthesis; Cell cycle; Cell Cycle Checkpoints - drug effects; Damage; Deactivation; Deoxyribonucleic acid; DNA; DNA Damage; DNA glycosylase; DNA repair; Fungi; Genes; Genetic aspects; Genomes; Homologous recombination; Homology; Hygromycin; Hygromycin B; Hygromycin B - pharmacology; Inactivation; Inhibitors; Kluyveromyces lactis; Lesions; Medicine and Health Sciences; Mutagenesis; Mutants; Mutation; Non-homologous end joining; Nuclease; Physiological aspects; Pichia; Plasmids; Protein biosynthesis; Protein Biosynthesis - drug effects; Repair; Research and Analysis Methods; Ribonucleases - metabolism; Ribonucleotide reductase; RNA, Fungal - genetics; RNA, Transfer - metabolism; Saccharomyces cerevisiae; Toxicity; Toxins; Transfer RNA; Translation; tRNA; Yeast; Yeasts - enzymology; Yeasts - genetics; Yeasts - growth & development; Yeasts - metabolism; Germany

Previous studies revealed DNA damage to occur during the toxic action of PaT, a fungal anticodon ribonuclease (ACNase) targeting the translation machinery via tRNA cleavage. Here, we demonstrate that other translational stressors induce DNA damage-like responses in yeast as well: not only zymocin, a...