Gut dysbiosis is associated with metabolism and systemic inflammation in patients with ischemic stroke

• Published in: PloS one Vol. 12; no. 2; p. e0171521
• Main Authors: Yamashiro, Kazuo, Tanaka, Ryota, Urabe, Takao, Ueno, Yuji, Yamashiro, Yuichiro, Nomoto, Koji, Takahashi, Takuya, Tsuji, Hirokazu, Asahara, Takashi, Hattori, Nobutaka
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.02.2017; Public Library of Science (PLoS)
• Subjects: Acetic acid; Antibiotics; Atherosclerosis; Autoimmune diseases; Bacteria; Biology and Life Sciences; Brain research; C-reactive protein; Cardiovascular disease; Cholesterol; Chromatography; Complications and side effects; Correlation; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Dietary fiber; Dysbacteriosis; Dysbiosis; Enzymes; Fatty acids; Feces; Fermentation; Glucose; Hemoglobin; High performance liquid chromatography; Hospitals; Hypertension; Inflammation; Interleukin 6; Intestinal microflora; Ischemia; Laboratories; Leukocytes; Liquid chromatography; Medicine; Medicine and Health Sciences; Metabolic disorders; Metabolism; Metabolites; Microbiota; Microbiota (Symbiotic organisms); Multivariable control; Neurology; Obesity; Organic acids; Pathogenesis; Patients; Physical Sciences; Probiotics; Proteins; Regression analysis; Reverse transcription; Risk factors; rRNA 16S; rRNA 23S; Stroke; Tumor necrosis factor-TNF; University graduates; Valeric acid; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0171521

The role of metabolic diseases in ischemic stroke has become a primary concern in both research and clinical practice. Increasing evidence suggests that dysbiosis is associated with metabolic diseases. The aim of this study was to investigate whether the gut microbiota, as well as concentrations of organic acids, the major products of dietary fiber fermentation by the gut microbiota, are altered in patients with ischemic stroke, and to examine the association between these changes and host metabolism and inflammation. We analyzed the composition of the fecal gut microbiota and the concentrations of fecal organic acids in 41 ischemic stroke patients and 40 control subjects via 16S and 23S rRNA-targeted quantitative reverse transcription (qRT)-PCR and high-performance liquid chromatography analyses, respectively. Multivariable linear regression analysis was subsequently performed to evaluate the relationships between ischemic stroke and bacterial counts and organic acid concentrations. Correlations between bioclinical markers and bacterial counts and organic acids concentrations were also evaluated. Although only the bacterial counts of Lactobacillus ruminis were significantly higher in stroke patients compared to controls, multivariable analysis showed that ischemic stroke was independently associated with increased bacterial counts of Atopobium cluster and Lactobacillus ruminis, and decreased numbers of Lactobacillus sakei subgroup, independent of age, hypertension, and type 2 diabetes. Changes in the prevalence of Lactobacillus ruminis were positively correlated with serum interleukin-6 levels. In addition, ischemic stroke was associated with decreased and increased concentrations of acetic acid and valeric acid, respectively. Meanwhile, changes in acetic acid concentrations were negatively correlated with the levels of glycated hemoglobin and low-density lipoprotein cholesterol, whereas changes in valeric acid concentrations were positively correlated with the level of high sensitivity C-reactive protein and with white blood cell counts. Together, our findings suggest that gut dysbiosis in patients with ischemic stroke is associated with host metabolism and inflammation.