The Effects of Hormones and Vaginal Microflora on the Glycome of the Female Genital Tract: Cervical-Vaginal Fluid

• Published in: PloS one Vol. 11; no. 7; p. e0158687
• Main Authors: Moncla, Bernard J., Chappell, Catherine A., Debo, Brian M., Meyn, Leslie A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.07.2016; Public Library of Science (PLoS)
• Subjects: Acids; alpha-Galactosidase - metabolism; Bacteria; beta-Galactosidase - metabolism; Binding sites; Biology; Biology and Life Sciences; Body Fluids - enzymology; Carbohydrates; Cell adhesion & migration; Cervix; Cervix mucus; Cervix Mucus - enzymology; Contraception; Data interpretation; Data processing; Environmental changes; Enzymes; Female; Female genitalia; Females; Galactosidase; Gardnerella vaginalis; Genital tract; Genitalia, Female - enzymology; Genitalia, Female - microbiology; Genitalia, Female - pathology; Glucosidase; Glycosidases; Glycoside Hydrolases - metabolism; Gynecology; Hormones; Hormones - metabolism; Humans; Immunity; Influenza; Innate immunity; Lectins; Lectins - pharmacology; Mannose; Medicine and Health Sciences; Menstrual cycle; Menstrual Cycle - metabolism; Microbiota; Microorganisms; Monitoring methods; Mucin; Mucin-1 - metabolism; Mucin-4 - metabolism; Mucin-5B - metabolism; Mucins; Mucins - metabolism; Mucus; N-Acetylneuraminic Acid - metabolism; Neuraminidase - metabolism; Obstetrics; Pathogenesis; Pathogens; Physical Sciences; Physiological aspects; Post-menopause; Proteins; Quantitation; Reproductive system; Salivary Proteins and Peptides - metabolism; Sambucus nigra; Vagina; Vagina - metabolism; Vagina - microbiology; Vagina - pathology; Vaginal discharge; Vaginosis; Vaginosis, Bacterial - enzymology; Vaginosis, Bacterial - microbiology; Vaginosis, Bacterial - pathology; Viruses; Viscoelasticity; α-Glucosidase; β-Galactosidase; United States

In this study, we characterized the glycome of cervical-vaginal fluid, collected with a Catamenial cup. We quantified: glycosidase levels; sialic acid and high mannose specific lectin binding; mucins, MUC1, MUC4, MUC5AC, MUC7; and albumin in the samples collected. These data were analyzed in the con...