Evidence-Based Annotation of Gene Function in Shewanella oneidensis MR-1 Using Genome-Wide Fitness Profiling across 121 Conditions

• Published in: PLoS genetics Vol. 7; no. 11; p. e1002385
• Main Authors: Deutschbauer, Adam, Price, Morgan N., Wetmore, Kelly M., Shao, Wenjun, Baumohl, Jason K., Xu, Zhuchen, Nguyen, Michelle, Tamse, Raquel, Davis, Ronald W., Arkin, Adam P.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.11.2011; Public Library of Science (PLoS)
• Subjects: Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; BASIC BIOLOGICAL SCIENCES; Biology; DNA sequencing; DNA Transposable Elements - genetics; E coli; Experiments; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genetic aspects; Genetic Association Studies; Genetic Fitness - genetics; Genetics; Genetics & Heredity; Genome, Bacterial; Genomes; Genomics; Molecular Sequence Annotation; Multigene Family - genetics; Mutagenesis; Mutagenesis - genetics; Mutation; Nucleic acid probes; Nucleotide sequencing; Oligonucleotide Array Sequence Analysis; Operon - genetics; Phenotype; Physiological aspects; Protein kinases; Shewanella; Shewanella - genetics; Signal Transduction; United States

Most genes in bacteria are experimentally uncharacterized and cannot be annotated with a specific function. Given the great diversity of bacteria and the ease of genome sequencing, high-throughput approaches to identify gene function experimentally are needed. Here, we use pools of tagged transposon...