RNA Interference of Four Genes in Adult Bactrocera dorsalis by Feeding Their dsRNAs

• Published in: PloS one Vol. 6; no. 3; p. e17788
• Main Authors: Li, Xiaoxue, Zhang, Mingyan, Zhang, Hongyu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.03.2011; Public Library of Science (PLoS)
• Subjects: Adenosine triphosphatase; Adenosinetriphosphatase; Agriculture; Animals; ATPases; Bacteria; Bactrocera dorsalis; Bioassays; Biology; Cloning, Molecular; Comparative analysis; Diptera; Double-stranded RNA; Drosophila; E coli; Egg production; Experiments; Fat body; Fatty acids; Feeding; Feeding Behavior; Gene expression; Gene Knockdown Techniques; Gene silencing; Genes; Genes, Insect; Genetic engineering; Genetic research; Genetically modified organisms; Genomes; Guanosine triphosphatases; Hepatitis; Hepatitis delta virus; Horticulture; Influenza; Ingestion; Insects; Interference; Interferon; Laboratories; Midgut; Nervous system; Overexpression; Pest control; Plant sciences; Proteins; Reproduction; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleic acid; RNA; RNA Interference; RNA, Double-Stranded - genetics; RNA-mediated interference; Tephritidae - genetics; Tephritidae - physiology; Testes; Tissues; Viruses; New York; United States > US; China

RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner. Recently silencing the target gene through feeding has been successfully carried out in many insect species. Escherichia coli strain HT115 was genetically engineered to express dsRNA targeting gene...