Quantitative assessment of the sensitivity of various commercial reverse transcriptases based on armored HIV RNA

• Published in: PloS one Vol. 5; no. 11; p. e13931
• Main Authors: Okello, John B A, Rodriguez, Linda, Poinar, Debi, Bos, Kirsten, Okwi, Andrew L, Bimenya, Gabriel S, Sewankambo, Nelson K, Henry, Kenneth R, Kuch, Melanie, Poinar, Hendrik N
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.11.2010; Public Library of Science (PLoS)
• Subjects: Cell Biology/Gene Expression; Comparative analysis; Complementary DNA; Copy number; Deoxyribonucleic acid; DNA; DNA polymerase; DNA polymerases; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; Efficiency; Enzymes; Foot & mouth disease; Gene expression; Genetic transcription; Genetics and Genomics; genomics; Health sciences; HIV; HIV (Viruses); HIV - genetics; Human immunodeficiency virus; Humans; Lysates; Molecular Biology; nucleic acids; Paraffin; Pathology; Polymerase chain reaction; Purification; Rankings; Reproducibility; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse transcription; Reverse Transcription - genetics; Ribonucleic acid; RNA; RNA, Viral - genetics; RNA, Viral - metabolism; RNA-directed DNA polymerase; RNA-Directed DNA Polymerase - metabolism; Sensitivity; Sensitivity analysis; Studies; Tissues; Trends; Virology/Diagnosis; Ontario Canada; Canada; Uganda; Kampala Uganda

The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as possible, especi...