A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana

• Published in: PLoS genetics Vol. 16; no. 12; p. e1008983
• Main Authors: Ghoshal, Basudev, Vong, Brandon, Picard, Colette L., Feng, Suhua, Tam, Janet M., Jacobsen, Steven E.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.12.2020; Public Library of Science (PLoS)
• Subjects: Arabidopsis; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biology and life sciences; CRISPR; CRISPR-Cas Systems; Demethylation; Deoxyribonucleic acid; Diseases and pests; Distribution; DNA; DNA Methylation; Drug delivery systems; Drugs; Engineering and Technology; Epigenetics; Epigenome; Experiments; Gene Editing - methods; Gene expression; Gene regulation; Gene Targeting - methods; Genetic aspects; Genomes; Innovations; Plant Viruses - genetics; Proteins; Repressors; Research and Analysis Methods; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - genetics; RNA, Transfer - genetics; Tobacco mosaic virus; Transcription activation; Transcription factors; Transcriptional Activation; Transcriptional coactivators; Transgenic plants; Vectors (Biology); Vehicles; Viruses; United States

Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication and movement. Utilizing this concept, several viral-based guide RNA delivery platforms for CRISPR-Cas9 genome editing have been developed. The CRISPR-Cas9 system has a...