The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation

• Published in: The EMBO journal Vol. 20; no. 21; pp. 5971 - 5981
• Main Authors: Suzuki, Kuninori, Kirisako, Takayoshi, Kamada, Yoshiaki, Mizushima, Noboru, Noda, Takeshi, Ohsumi, Yoshinori
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.11.2001; Nature Publishing Group UK; Springer Nature B.V; Oxford University Press
• Subjects: APG; APG gene; Apg1 protein; Apg12 protein; Apg16 protein; Apg5 protein; Apg9 protein; Aut7 protein; autophagosome; autophagosomes; Autophagy; Autophagy - physiology; Autophagy-Related Protein 5; Autophagy-Related Protein 8 Family; Autophagy-Related Proteins; Carrier Proteins; Carrier Proteins - genetics; Carrier Proteins - metabolism; cytochemistry; Fungal Proteins; Fungal Proteins - genetics; Fungal Proteins - metabolism; genes; genetic techniques and protocols; genetics; GFP; Green Fluorescent Proteins; Heat-Shock Proteins; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Luminescent Proteins; Luminescent Proteins - genetics; macroautophagy; Macromolecular Substances; membrane dynamics; Membrane Proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; metabolism; Microtubule-Associated Proteins; Microtubule-Associated Proteins - metabolism; Mutagenesis; mutants; phagocytosis; phosphatidylethanolamine; physiology; Protein Processing, Post-Translational; Protein Processing, Post-Translational - physiology; Proteins; Recombinant Fusion Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Temperature; Transcription Factors; Ubiquitin-Protein Ligases; vacuoles; Vacuoles - metabolism; Vesicular Transport Proteins; Yeasts; GFP; APG; autophagy; autophagosome; membrane dynamics

Macroautophagy is a bulk degradation process induced by starvation in eukaryotic cells. In yeast, 15 Apg proteins coordinate the formation of autophagosomes. Several key reactions performed by these proteins have been described, but a comprehensive understanding of the overall network is still lacki...