A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

• Published in: PloS one Vol. 14; no. 1; p. e0210243
• Main Authors: Leynaud-Kieffer, Laure M. C., Curran, Samuel C., Kim, Irene, Magnuson, Jon K., Gladden, John M., Baker, Scott E., Simmons, Blake A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.01.2019; Public Library of Science (PLoS)
• Subjects: Aspergillus; Aspergillus niger; Aspergillus niger - genetics; BASIC BIOLOGICAL SCIENCES; Biodiesel fuels; Biology and Life Sciences; Biotechnology; CRISPR; CRISPR-Cas Systems - genetics; Deoxyribonucleic acid; DNA; Efficiency; Engineering and Technology; Enzymes; Fungi; Gene Editing - methods; Gene expression; Gene Targeting; Genetic aspects; Genetic engineering; Genetic modification; Genome editing; Genome, Fungal - genetics; Genomes; Genomics; Laboratories; Loci; Metabolic pathways; Mutation; Optimization; Proteins; Recombination; Research and Analysis Methods; Secretion; United States > US; California; Pacific Northwest

Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic engineering of these hosts remains nascent. For example, while molecular genetic tools have been developed, including CRISPR/Cas9, facile genome engineering of A. niger remains challenging. To address th...