Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling

• Published in: Fertility and sterility Vol. 100; no. 5; pp. 1468 - 1475.e2
• Main Authors: Park, Eun-Sil, Woods, Dori C., Tilly, Jonathan L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2013
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; adult stem cells; Adult Stem Cells - drug effects; Adult Stem Cells - metabolism; adults; animal ovaries; Animals; antagonists; BMP4; Bone Morphogenetic Protein 4 - pharmacology; Bone Morphogenetic Protein Receptors - genetics; Bone Morphogenetic Protein Receptors - metabolism; bone morphogenetic proteins; Carrier Proteins - pharmacology; cell differentiation; Cells, Cultured; Dose-Response Relationship, Drug; Female; flow cytometry; gene expression; genes; germ cells; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Internal Medicine; meiosis; Mice; Mice, Inbred C57BL; MSX1 Transcription Factor - genetics; MSX1 Transcription Factor - metabolism; Obstetrics and Gynecology; oocyte; oocytes; Oocytes - drug effects; Oocytes - metabolism; oogenesis; Oogenesis - drug effects; Phosphorylation; Recombinant Proteins - pharmacology; retinoic acid; Signal Transduction - drug effects; Smad1 Protein - metabolism; Smad5 Protein - metabolism; Smad8 Protein - metabolism; stem cells; Time Factors; transcriptional activation; BMP4; stem cells; oocyte; germ cells; oogenesis

To test whether bone morphogenetic protein 4 (BMP4) directly regulates differentiation of adult mouse ovary-derived oogonial stem cells (OSCs) in vitro. Animal study. Research laboratory. Adult C57BL/6 female mice. After purification from adult ovaries by fluorescence-activated cell sorting, OSCs we...