Expression of naturally ionic liquid-tolerant thermophilic cellulases in Aspergillus niger

• Published in: PloS one Vol. 12; no. 12; p. e0189604
• Main Authors: Amaike Campen, Saori, Lynn, Jed, Sibert, Stephanie J., Srikrishnan, Sneha, Phatale, Pallavi, Feldman, Taya, Guenther, Joel M., Hiras, Jennifer, Tran, Yvette Thuy An, Singer, Steven W., Adams, Paul D., Sale, Kenneth L., Simmons, Blake A., Baker, Scott E., Magnuson, Jon K., Gladden, John M.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.12.2017; Public Library of Science (PLoS)
• Subjects: 09 BIOMASS FUELS; Acetic acid; Alternative energy sources; Aspergillus; Aspergillus niger; Bacteria; Biodiesel fuels; Biofuels; Biology and Life Sciences; Biomass; Biotechnology; Cellulase; Cellulose; Costs; Deconstruction; E coli; Earth science; Ecology and Environmental Sciences; Electrons; Engineering; Engineering and Technology; Enzymatic activity; Enzymes; Escherichia coli; Gene expression; Genetic aspects; Genomics; Geochemistry; Glucosidase; Hydrolases; Hydrolysis; Ionic liquids; Laboratories; Lignocellulose; Panicum virgatum; Physical Sciences; Physiological aspects; Plant biomass; Production processes; Proteins; Research and Analysis Methods; Sugar; United States

Efficient deconstruction of plant biomass is a major barrier to the development of viable lignocellulosic biofuels. Pretreatment with ionic liquids reduces lignocellulose recalcitrance to enzymatic hydrolysis, increasing yields of sugars for conversion into biofuels. However, commercial cellulases a...