Bacteriorhodopsin: Would the real structural intermediates please stand up?

• Published in: Biochimica et biophysica acta Vol. 1850; no. 3; pp. 536 - 553
• Main Authors: Wickstrand, Cecilia, Dods, Robert, Royant, Antoine, Neutze, Richard
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2015; Elsevier
• Subjects: 2.3 ANGSTROM RESOLUTION; annealing; aspartic acid; Bacteriorhodopsin; Bacteriorhodopsins - chemistry; Bacteriorhodopsins - classification; Bacteriorhodopsins - genetics; Biochemistry & Molecular Biology; Biofysik; Biophysics; CONFORMATIONAL-CHANGES; crystallization; CRYSTALLIZING MEMBRANE-PROTEINS; CRYSTALLOGRAPHIC; Crystallography, X-Ray; crystals; ELECTRON CRYSTALLOGRAPHY; Global structural analysis; Life Sciences; Light; LIPIDIC CUBIC PHASES; Models, Molecular; Molecular Biology; Molekylärbiologi; mutagenesis; nuclear magnetic resonance spectroscopy; Phylogeny; Protein Conformation - radiation effects; Protein Structure, Secondary - radiation effects; proton pump; Proton pumping; RADIATION-DAMAGE; schiff bases; SCHIFF-BASE; Structural Biology; Structural intermediates; STRUCTURE; TRANSPORT; trees; VECTORIAL PROTON; X-radiation; X-ray diffraction; X-RAY-STRUCTURE; X-Rays; Bacteriorhodopsin; Global structural analysis; Proton pumping; Structural intermediates

Bacteriorhodopsin (bR) is the simplest known light driven proton pump and has been heavily studied using structural methods: eighty four X-ray diffraction, six electron diffraction and three NMR structures of bR are deposited within the protein data bank. Twenty one X-ray structures report light ind...