Allele specific expression of Dof genes responding to hormones and abiotic stresses in sugarcane

• Published in: PloS one Vol. 15; no. 1; p. e0227716
• Main Authors: Cai, Mingxing, Lin, Jishan, Li, Zeyun, Lin, Zhicong, Ma, Yaying, Wang, Yibin, Ming, Ray
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.01.2020; Public Library of Science (PLoS)
• Subjects: Abiotic stress; Alleles; Barley; BASIC BIOLOGICAL SCIENCES; Biology; Biology and Life Sciences; Biotechnology; Criminal investigation; Deoxyribonucleic acid; DNA; DNA binding proteins; Engineering and Technology; ENVIRONMENTAL SCIENCES; Ethanol; Gene Duplication - physiology; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - physiology; Genes; Genetic aspects; Genome, Plant - genetics; Genomes; Genomics; Hormones; Laboratories; Life sciences; Phylogenetics; Phylogeny; Plant Growth Regulators - pharmacology; Plant hormones; Plant Proteins - genetics; Plant Proteins - metabolism; Profiling; Proteins; Research and Analysis Methods; Rice; Saccharum - physiology; Science & Technology - Other Topics; Software; Stress, Physiological - genetics; Stresses; Sugarcane; Transcription (Genetics); Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0227716

Dof transcription factors plant-specific and associates with growth and development in plants. We conducted comprehensive and systematic analyses of Dof transcription factors in sugarcane, and identified 29 SsDof transcription factors in sugarcane genome. Those SsDof genes were divided into five groups, with similar gene structures and conserved motifs within the same groups. Segmental duplications are predominant in the evolution of Dof in sugarcane. Cis-element analysis suggested that the functions of SsDofs were involved in growth and development, hormones and abiotic stresses responses in sugarcane. Expression patterns indicated that SsDof7, SsDof23 and SsDof24 had a comparatively high expression in all detected tissues, indicating these genes are crucial in sugarcane growth and development. Moreover, we examined the transcription levels of SsDofs under four plant hormone treatments, SsDof7-3 and SsDof7-4 were down-regulated after ABA treatment, while SsDof7-1 and SsDof7-2 were induced after the same treatment, indicating different alleles may play different roles in response to plant hormones. We also analyzed SsDofs' expression profiling under four abiotic stresses, SsDof5 and SsDof28 significantly responded to these four stresses, indicating they are associate with abiotic stresses responses. Collectively, our results yielded allele specific expression of Dof genes responding to hormones and abiotic stresses in sugarcane, and their cis-elements could be crucial for sugarcane improvement.