Gene‐specific correlation of RNA and protein levels in human cells and tissues

• Published in: Molecular systems biology Vol. 12; no. 10; pp. 883 - n/a
• Main Authors: Edfors, Fredrik, Danielsson, Frida, Hallström, Björn M, Käll, Lukas, Lundberg, Emma, Pontén, Fredrik, Forsström, Björn, Uhlén, Mathias
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2016; EMBO Press; John Wiley and Sons Inc; Springer Nature
• Subjects: Biotechnology; Bioteknologi; Cell Line; Cell lines; Consortia; Deoxyribonucleic acid; DNA; EMBO17; EMBO31; EMBO44; Gene Expression; Gene Expression Profiling - methods; Genes; Genomes; Genomics; Human tissues; Humans; Laboratories; Molecular biology; Peptides; protein quantification; Proteins; Proteome - genetics; Proteome - metabolism; Proteomics; Proteomics - methods; Ribonucleic acid; RNA; Studies; targeted proteomics; Tissues; Transcription; transcriptomics; targeted proteomics; transcriptomics; protein quantification; gene expression

An important issue for molecular biology is to establish whether transcript levels of a given gene can be used as proxies for the corresponding protein levels. Here, we have developed a targeted proteomics approach for a set of human non‐secreted proteins based on parallel reaction monitoring to mea...