Paracrine regulation of theca androgen production by granulosa cells in the ovary

• Published in: Fertility and sterility Vol. 100; no. 2; pp. 561 - 567
• Main Authors: Hoang, Yvonne D., McTavish, Kirsten J., Chang, R. Jeffrey, Shimasaki, Shunichi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2013
• Subjects: Androgens - metabolism; Animals; antibodies; Aromatase - genetics; Aromatase - metabolism; Cells, Cultured; Cyp17; explants; Female; Follicle Stimulating Hormone - pharmacology; follicle-stimulating hormone; gene expression; Gene Expression Regulation, Enzymologic - drug effects; granulosa cell; granulosa cells; Granulosa Cells - drug effects; Granulosa Cells - metabolism; in vitro studies; inhibin; Internal Medicine; messenger RNA; Obstetrics and Gynecology; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; Ovarian Follicle - physiology; Ovary - drug effects; Ovary - metabolism; Ovary - physiology; Paracrine Communication - drug effects; Paracrine Communication - genetics; Paracrine Communication - physiology; polycystic ovary syndrome; Primary Cell Culture; Rats; Rats, Sprague-Dawley; reverse transcriptase polymerase chain reaction; Steroid 17-alpha-Hydroxylase - genetics; Steroid 17-alpha-Hydroxylase - metabolism; Theca cell; Theca Cells - drug effects; Theca Cells - metabolism; Theca Cells - physiology; Cyp17; granulosa cell; Theca cell; inhibin; polycystic ovary syndrome

To test whether and to what extent inhibin mediates Cyp17 messenger RNA (mRNA) expression in theca cells (TCs) in response to FSH stimulation of granulosa cells (GCs). Ex vivo and in vitro experimental study. University. Immature female Sprague Dawley rats. Ovarian tissue explants and isolated theca...