The detection of bovine lactoferrin binding protein on Toxoplasma gondii

• Published in: Journal of Veterinary Medical Science Vol. 65; no. 12; pp. 1377 - 1380
• Main Authors: Tanaka, T. (Hokkaido Univ., Sapporo (Japan)), Abe, Y, Kim, W.S, Xuan, X, Nagasawa, H, Igarashi, I, Kumura, H, Shimazaki, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.12.2003; Japan Science and Technology Agency
• Subjects: Animals; Bacterial Proteins; Binding, Competitive; Carrier Proteins - analysis; Cattle; Conalbumin - metabolism; DOMESTIC ANIMALS; IMMUNOLOGICAL TECHNIQUES; LACTOFERRIN; Lactoferrin - metabolism; Microscopy, Fluorescence; Protein Binding; Protozoan Proteins - analysis; Toxoplasma - cytology; TOXOPLASMA GONDII; transferrin family protein; TRANSFERRINS
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.65.1377

Lactoferrin (LF), a member of the transferrin (TF) protein family, is an iron-binding protein that is known to interact with bacteria through a specific receptor. We examined the binding of bovine LF (bLF), bovine TF (bTF), and ovotransferrin (OTF) by Toxoplasma gondii using a fluorescence test and the streptavidin-biotin (SAB) method using biotin-streptavidin, and found that bLF, bTF, and OTF bound to the protein components of T. gondii. Furthermore, we confirmed that bLF, bTF, and OTF bound a 42 kDa soluble protein of T. gondii by far Western blot method. These results demonstrated that bLF binding proteins are present on T. gondii.