Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp
Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biom...
Saved in:
| Published in | Biotechnology for biofuels Vol. 12; no. 1; p. 39 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
BioMed Central Ltd
21.02.2019
BioMed Central BMC |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga
sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions.
Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO
under the LD condition, indicating higher CO
fixation than that of the LL condition.
Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO
fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. |
|---|---|
| AbstractList | Background Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. Results Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO2 under the LD condition, indicating higher CO2 fixation than that of the LL condition. Conclusions Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. Background Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. Results Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO.sub.2 under the LD condition, indicating higher CO.sub.2 fixation than that of the LL condition. Conclusions Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO.sub.2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. Keywords: Biofuel, Biomass, Chlamydomonas, Light/dark cycling, Lipid, Metabolic profiling, Microalgae, Photoperiod, Carbohydrate Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions.BACKGROUNDLight/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions.Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO2 under the LD condition, indicating higher CO2 fixation than that of the LL condition.RESULTSLipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO2 under the LD condition, indicating higher CO2 fixation than that of the LL condition.Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production.CONCLUSIONSPhotoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. Abstract Background Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. Results Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO2 under the LD condition, indicating higher CO2 fixation than that of the LL condition. Conclusions Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. BACKGROUND: Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. RESULTS: Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO₂ under the LD condition, indicating higher CO₂ fixation than that of the LL condition. CONCLUSIONS: Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO₂ fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga Chlamydomonas sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO.sub.2 under the LD condition, indicating higher CO.sub.2 fixation than that of the LL condition. Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO.sub.2 fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The general aim of this study was to determine the influence of light/dark cycling on microalgal biomass production and lipid accumulation. To achieve this goal, specific causative mechanisms were investigated using a metabolomics approach. Laboratory scale photoautotrophic cultivations of the oleaginous green microalga sp. JSC4 were performed under continuous light (LL) and light/dark (LD) conditions. Lipid accumulation and carbohydrate degradation were delayed under the LD condition compared with that under the LL condition. Metabolomic analysis revealed accumulation of phosphoenolpyruvate and decrease of glycerol 3-phosphate under the LD condition, suggesting that the imbalance of these metabolites is a source of delayed lipid accumulation. When accounting for light dosage, biomass yield under the LD condition was significantly higher than that under the LL condition. Dynamic metabolic profiling showed higher levels of lipid/carbohydrate anabolism (including production of 3-phosphoglycerate, fructose 6-phosphate, glucose 6-phosphate, phosphoenolpyruvate and acetyl-CoA) from CO under the LD condition, indicating higher CO fixation than that of the LL condition. Photoperiods define lipid accumulation and biomass production, and light/dark cycling was determined as a critical obstacle for lipid production in JSC4. Conversions of phosphoenolpyruvate to pyruvate and 3-phosphoglycerate to glycerol 3-phosphate are the candidate rate-limiting steps responsible for delayed lipid accumulation. The accumulation of substrates including ribulose 5-phosphate could be explained by the close relationship of increased biomass yield with enhanced CO fixation. The present study investigated the influence of light/dark cycling on lipid production by direct comparison with continuous illumination for the first time, and revealed underlying metabolic mechanisms and candidate metabolic rate-limiting steps during light/dark cycling. These findings suggest promising targets to metabolically engineer improved lipid production. |
| ArticleNumber | 39 |
| Audience | Academic |
| Author | Fujihara, Yusuke Chang, Jo-Shu Kondo, Akihiko Hasunuma, Tomohisa Vavricka, Christopher J. Kato, Yuichi |
| Author_xml | – sequence: 1 givenname: Yuichi surname: Kato fullname: Kato, Yuichi – sequence: 2 givenname: Yusuke surname: Fujihara fullname: Fujihara, Yusuke – sequence: 3 givenname: Christopher J. surname: Vavricka fullname: Vavricka, Christopher J. – sequence: 4 givenname: Jo-Shu surname: Chang fullname: Chang, Jo-Shu – sequence: 5 givenname: Tomohisa surname: Hasunuma fullname: Hasunuma, Tomohisa – sequence: 6 givenname: Akihiko surname: Kondo fullname: Kondo, Akihiko |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30828384$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkktr3DAUhU1JaR7tD-imGLppF04ky9ZjUwhDH4FAoY-1uJZkj1JZmlp2yfyX_tjeZNKQyaLFC9v3fucgHc5xcRBTdEXxkpJTSiU_y5QRLitCVUWZJFXzpDiiom0qLllz8OD7sDjO-YoQTgURz4pDRmQtmWyOit-XfljPZxamH6XZmuDjUBpYssuldQG2zpbBb7wtwZhlXALMPsUSoi19NJODjMBmnea0cZNPtupuJ51PI-Rcbr0L-LctIcy4R-_RzdCl4E3Zh-UaTcoUHAw-piWXq3WAcWvTmCLkMm-eF097CNm9uHufFN8_vP-2-lRdfv54sTq_rIwgaq4sU9R1rq37TjLR4oV70ffKSFIzxTuqjGoksQR6YUDUhEHNDLcEdy2VDthJcbHztQmu9GbyI0xbncDr20GaBg3T7E1wmqvOQdN1xqIpddBRqJXhXPSs7VRbo9e7nddm6UZnjYvzBGHPdH8T_VoP6ZfmTLJaEDR4c2cwpZ-Ly7MefTYuBIgOQ9J1o2TNhWrq_6NUCsUJtgTR14_Qq7RMEVNFSjHRNC3hSJ3uqAHwrj72CY9o8LFu9AbL13ucn7eSYuS1YCh4uydAZnbX84ANyvri65d99tXDZO4j-VtGBOgOMFPKeXL9PUKJvim83hVeY-H1TeH1jUY80hg_35YUT-7DP5R_AJQfBrU |
| CitedBy_id | crossref_primary_10_1016_j_biteb_2023_101722 crossref_primary_10_1016_j_rser_2021_111270 crossref_primary_10_1016_j_envres_2023_116533 crossref_primary_10_1111_pre_12441 crossref_primary_10_1016_j_algal_2023_103144 crossref_primary_10_1016_j_algal_2020_102021 crossref_primary_10_1007_s00253_024_13199_y crossref_primary_10_1016_j_jenvman_2022_115225 crossref_primary_10_1016_j_biochi_2019_09_017 crossref_primary_10_1016_j_scitotenv_2022_159920 crossref_primary_10_1016_j_fuel_2020_118440 crossref_primary_10_1021_acssynbio_9b00281 crossref_primary_10_1016_j_scitotenv_2023_169082 crossref_primary_10_3390_life12030462 crossref_primary_10_1007_s00604_024_06662_0 crossref_primary_10_1007_s10811_021_02416_0 crossref_primary_10_1186_s13068_019_1493_9 crossref_primary_10_3390_su13126606 crossref_primary_10_1016_j_cej_2024_149619 crossref_primary_10_1016_j_biortech_2021_126196 crossref_primary_10_3389_fbioe_2023_1102098 crossref_primary_10_1038_s41540_022_00260_7 crossref_primary_10_1038_s42003_021_01976_8 crossref_primary_10_1016_j_rser_2024_114877 crossref_primary_10_1016_j_eti_2025_104138 crossref_primary_10_1111_1442_1984_12403 crossref_primary_10_3390_en16052429 crossref_primary_10_1007_s13762_025_06419_z crossref_primary_10_1002_cjce_24116 |
| Cites_doi | 10.1155/2015/519513 10.1186/s13068-018-1181-1 10.1023/A:1020661719272 10.1186/s13068-017-0762-8 10.1038/nbt.2489 10.1038/srep45471 10.1016/j.biortech.2016.11.004 10.1371/journal.pone.0127562 10.1016/j.biortech.2018.01.020 10.1139/y59-099 10.1186/s13068-017-0776-2 10.1046/j.1529-8817.2001.01052.x 10.1038/nature11479 10.1016/j.biortech.2018.03.002 10.1016/j.biortech.2013.08.162 10.1016/j.algal.2015.10.012 10.1186/1754-6834-7-97 10.1016/j.biotechadv.2007.02.001 10.1186/s13068-015-0226-y 10.1104/pp.106.081885 10.1002/biot.201500284 10.1007/s10499-011-9440-1 10.1016/j.biortech.2017.06.035 10.1073/pnas.1504576112 10.1023/A:1008046023487 10.1111/tpj.13642 10.1016/j.jbiotec.2015.04.013 10.1080/09670269910001736332 10.1016/j.biortech.2012.11.032 10.1007/s00425-010-1282-y 10.1007/s00449-016-1633-6 10.1007/s00449-013-1044-x 10.1111/tpj.14078 10.1039/C4GC01612B 10.1186/s13068-016-0671-2 10.1016/j.biortech.2013.11.009 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2019 BioMed Central Ltd. Copyright © 2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Author(s) 2019 |
| Copyright_xml | – notice: COPYRIGHT 2019 BioMed Central Ltd. – notice: Copyright © 2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: The Author(s) 2019 |
| DBID | AAYXX CITATION NPM ISR 3V. 7QO 7SP 7ST 7TB 7X7 7XB 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. L6V L7M LK8 M0S M7P M7S P5Z P62 P64 PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PTHSS SOI 7X8 7S9 L.6 5PM DOA |
| DOI | 10.1186/s13068-019-1380-4 |
| DatabaseName | CrossRef PubMed Gale In Context: Science ProQuest Central (Corporate) Biotechnology Research Abstracts Electronics & Communications Abstracts Environment Abstracts Mechanical & Transportation Engineering Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Engineering Collection Advanced Technologies Database with Aerospace Biological Sciences ProQuest Health & Medical Collection Biological Science Database Engineering Database Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition Engineering Collection Environment Abstracts MEDLINE - Academic AGRICOLA AGRICOLA - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals - May need to register for free articles |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest Central Student Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Mechanical & Transportation Engineering Abstracts ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Engineering Collection Health Research Premium Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) Advanced Technologies Database with Aerospace Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Electronics & Communications Abstracts ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Advanced Technologies & Aerospace Database ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Materials Science & Engineering Collection Engineering Research Database ProQuest One Academic Environment Abstracts ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | Publicly Available Content Database MEDLINE - Academic AGRICOLA PubMed |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| EISSN | 1754-6834 2731-3654 |
| EndPage | 39 |
| ExternalDocumentID | oai_doaj_org_article_69bea4bbcdc941eab1a29c667f35b952 PMC6383270 A581391273 30828384 10_1186_s13068_019_1380_4 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: ; |
| GroupedDBID | 23N 2WC 2XV 5GY 5VS 6J9 7X7 8FE 8FG 8FH 8FI 8FJ AAFWJ AAHBH AAYXX ABDBF ABJCF ABUWG ACGFO ACGFS ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFKRA AFPKN AFRAH AHBYD AHMBA AHYZX ALIPV ALMA_UNASSIGNED_HOLDINGS AMKLP AMTXH AOIJS ARAPS BAPOH BAWUL BBNVY BCNDV BENPR BFQNJ BGLVJ BHPHI BPHCQ BVXVI CCPQU CITATION CS3 DIK DU5 E3Z EBS ECGQY EJD ESX F5P FYUFA GROUPED_DOAJ GX1 H13 HCIFZ HH5 HMCUK HYE I-F IAG IAO IEA IEP ISR ITC KQ8 L6V L8X LK8 M48 M7P M7S ML0 M~E O5R O5S OVT P2P P62 PGMZT PHGZM PHGZT PIMPY PQQKQ PROAC PTHSS RBZ RNS ROL RPM RVI SCM TR2 TUS UKHRP ~8M -A0 2VQ 3V. 4.4 ADINQ AHSBF BMC C1A C24 C6C IHR IPNFZ NPM OK1 RIG RSV SOJ PMFND 0R~ 7QO 7SP 7ST 7TB 7XB 8FD 8FK AAJSJ AASML ADUKV AZQEC C1K DWQXO EBLON FR3 GNUQQ K9. L7M P64 PKEHL PQEST PQGLB PQUKI SOI 7X8 7S9 L.6 5PM PUEGO |
| ID | FETCH-LOGICAL-c709t-d391ebe52fb8375683f7ff9c802396b19c9480d0af7ca7203a23c6d096b518ea3 |
| IEDL.DBID | 7X7 |
| ISSN | 1754-6834 |
| IngestDate | Wed Aug 27 01:28:14 EDT 2025 Thu Aug 21 18:10:22 EDT 2025 Thu Jul 10 17:03:27 EDT 2025 Fri Jul 11 07:16:17 EDT 2025 Sat Aug 23 12:39:39 EDT 2025 Tue Jun 10 20:47:45 EDT 2025 Fri Jun 27 04:22:30 EDT 2025 Thu Jan 02 22:54:12 EST 2025 Tue Jul 01 04:18:52 EDT 2025 Thu Apr 24 23:05:32 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Metabolic profiling Light/dark cycling Biofuel Carbohydrate Microalgae Photoperiod Biomass Chlamydomonas Lipid |
| Language | English |
| License | Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c709t-d391ebe52fb8375683f7ff9c802396b19c9480d0af7ca7203a23c6d096b518ea3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | https://www.proquest.com/docview/2193744506?pq-origsite=%requestingapplication% |
| PMID | 30828384 |
| PQID | 2193744506 |
| PQPubID | 55236 |
| PageCount | 1 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_69bea4bbcdc941eab1a29c667f35b952 pubmedcentral_primary_oai_pubmedcentral_nih_gov_6383270 proquest_miscellaneous_2498267942 proquest_miscellaneous_2187960130 proquest_journals_2193744506 gale_infotracacademiconefile_A581391273 gale_incontextgauss_ISR_A581391273 pubmed_primary_30828384 crossref_primary_10_1186_s13068_019_1380_4 crossref_citationtrail_10_1186_s13068_019_1380_4 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2019-02-21 |
| PublicationDateYYYYMMDD | 2019-02-21 |
| PublicationDate_xml | – month: 02 year: 2019 text: 2019-02-21 day: 21 |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: London |
| PublicationTitle | Biotechnology for biofuels |
| PublicationTitleAlternate | Biotechnol Biofuels |
| PublicationYear | 2019 |
| Publisher | BioMed Central Ltd BioMed Central BMC |
| Publisher_xml | – name: BioMed Central Ltd – name: BioMed Central – name: BMC |
| References | P Sirisuk (1380_CR33) 2018; 253 M Atta (1380_CR15) 2013; 148 JM Zones (1380_CR9) 2015; 27 YS Shin (1380_CR6) 2018; 258 S Jagadevan (1380_CR21) 2018; 11 Y Kato (1380_CR36) 2017; 245 A Nakanishi (1380_CR24) 2014; 152 C Banerjee (1380_CR5) 2016; 7 Y Chisti (1380_CR2) 2007; 25 H Wang (1380_CR18) 2016; 39 R Willamme (1380_CR13) 2015; 215 ZA Khoeyi (1380_CR34) 2012; 20 JP Ral (1380_CR12) 2006; 142 C Banerjee (1380_CR20) 2016; 11 S Lemaire (1380_CR10) 1999; 34 H Link (1380_CR25) 2013; 31 SR Medipally (1380_CR4) 2015; 2015 S Diamond (1380_CR26) 2015; 112 C Shene (1380_CR29) 2018; 96 J Fábregas (1380_CR30) 2002; 24 N Mallick (1380_CR1) 2016; 7 J Jüppner (1380_CR14) 2017; 92 SH Ho (1380_CR27) 2015; 8 SH Ho (1380_CR23) 2014; 7 L de Winter (1380_CR31) 2017; 10 Y Collos (1380_CR37) 1999; 11 KW Tan (1380_CR7) 2016; 9 JA Berges (1380_CR35) 2001; 37 DR Georgianna (1380_CR3) 2012; 488 LML Laurens (1380_CR39) 2015; 17 ÂP Matos (1380_CR17) 2017; 224 S Srirangan (1380_CR19) 2015; 10 M Vítová (1380_CR11) 2011; 233 S Wahidin (1380_CR16) 2013; 129 R Kumar (1380_CR22) 2017; 10 I Krzemińska (1380_CR8) 2014; 37 SJ Edmundson (1380_CR32) 2015; 12 EG Bligh (1380_CR38) 1959; 37 SH Ho (1380_CR28) 2017; 7 |
| References_xml | – volume: 2015 start-page: 519513 year: 2015 ident: 1380_CR4 publication-title: Biomed Res Int doi: 10.1155/2015/519513 – volume: 11 start-page: 185 year: 2018 ident: 1380_CR21 publication-title: Biotechnol Biofuels doi: 10.1186/s13068-018-1181-1 – volume: 24 start-page: 1699 year: 2002 ident: 1380_CR30 publication-title: Biotechnol Lett doi: 10.1023/A:1020661719272 – volume: 10 start-page: 104 year: 2017 ident: 1380_CR31 publication-title: Biotechnol Biofuels doi: 10.1186/s13068-017-0762-8 – volume: 31 start-page: 357 year: 2013 ident: 1380_CR25 publication-title: Nat Biotechnol doi: 10.1038/nbt.2489 – volume: 7 start-page: 45471 year: 2017 ident: 1380_CR28 publication-title: Sci Rep doi: 10.1038/srep45471 – volume: 224 start-page: 490 year: 2017 ident: 1380_CR17 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2016.11.004 – volume: 10 start-page: e0127562 year: 2015 ident: 1380_CR19 publication-title: PLoS ONE doi: 10.1371/journal.pone.0127562 – volume: 253 start-page: 175 year: 2018 ident: 1380_CR33 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2018.01.020 – volume: 37 start-page: 911 year: 1959 ident: 1380_CR38 publication-title: Can J Biochem Physiol doi: 10.1139/y59-099 – volume: 10 start-page: 94 year: 2017 ident: 1380_CR22 publication-title: Biotechnol Biofuels doi: 10.1186/s13068-017-0776-2 – volume: 37 start-page: 1138 year: 2001 ident: 1380_CR35 publication-title: J Phycol doi: 10.1046/j.1529-8817.2001.01052.x – volume: 27 start-page: 2743 year: 2015 ident: 1380_CR9 publication-title: Plant Cell – volume: 488 start-page: 329 year: 2012 ident: 1380_CR3 publication-title: Nature doi: 10.1038/nature11479 – volume: 258 start-page: 335 year: 2018 ident: 1380_CR6 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2018.03.002 – volume: 148 start-page: 373 year: 2013 ident: 1380_CR15 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2013.08.162 – volume: 12 start-page: 470 year: 2015 ident: 1380_CR32 publication-title: Algal Res doi: 10.1016/j.algal.2015.10.012 – volume: 7 start-page: 97 year: 2014 ident: 1380_CR23 publication-title: Biotechnol Biofuels doi: 10.1186/1754-6834-7-97 – volume: 25 start-page: 294 year: 2007 ident: 1380_CR2 publication-title: Biotechnol Adv doi: 10.1016/j.biotechadv.2007.02.001 – volume: 8 start-page: 48 year: 2015 ident: 1380_CR27 publication-title: Biotechnol Biofuels doi: 10.1186/s13068-015-0226-y – volume: 142 start-page: 305 year: 2006 ident: 1380_CR12 publication-title: Plant Physiol doi: 10.1104/pp.106.081885 – volume: 11 start-page: 303 year: 2016 ident: 1380_CR20 publication-title: J Biotechnol doi: 10.1002/biot.201500284 – volume: 20 start-page: 41 year: 2012 ident: 1380_CR34 publication-title: Aquacult Int doi: 10.1007/s10499-011-9440-1 – volume: 245 start-page: 1484 year: 2017 ident: 1380_CR36 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2017.06.035 – volume: 7 start-page: 432 year: 2016 ident: 1380_CR5 publication-title: Front Microbiol – volume: 112 start-page: E1916 year: 2015 ident: 1380_CR26 publication-title: Proc Natl Acad Sci USA. doi: 10.1073/pnas.1504576112 – volume: 11 start-page: 179 year: 1999 ident: 1380_CR37 publication-title: J Appl Phycol doi: 10.1023/A:1008046023487 – volume: 92 start-page: 331 year: 2017 ident: 1380_CR14 publication-title: Plant J doi: 10.1111/tpj.13642 – volume: 215 start-page: 20 year: 2015 ident: 1380_CR13 publication-title: J Biotechnol doi: 10.1016/j.jbiotec.2015.04.013 – volume: 34 start-page: 279 year: 1999 ident: 1380_CR10 publication-title: Eur J Phycol doi: 10.1080/09670269910001736332 – volume: 129 start-page: 7 year: 2013 ident: 1380_CR16 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2012.11.032 – volume: 233 start-page: 75 year: 2011 ident: 1380_CR11 publication-title: Planta doi: 10.1007/s00425-010-1282-y – volume: 39 start-page: 1589 year: 2016 ident: 1380_CR18 publication-title: Bioprocess Biosyst Eng doi: 10.1007/s00449-016-1633-6 – volume: 37 start-page: 735 year: 2014 ident: 1380_CR8 publication-title: Bioprocess Biosyst Eng doi: 10.1007/s00449-013-1044-x – volume: 7 start-page: 1019 year: 2016 ident: 1380_CR1 publication-title: Front Microbiol – volume: 96 start-page: 1076 year: 2018 ident: 1380_CR29 publication-title: Plant J doi: 10.1111/tpj.14078 – volume: 17 start-page: 1145 year: 2015 ident: 1380_CR39 publication-title: Green Chem doi: 10.1039/C4GC01612B – volume: 9 start-page: 255 year: 2016 ident: 1380_CR7 publication-title: Biotechnol Biofuels doi: 10.1186/s13068-016-0671-2 – volume: 152 start-page: 247 year: 2014 ident: 1380_CR24 publication-title: Bioresour Technol doi: 10.1016/j.biortech.2013.11.009 |
| SSID | ssj0061707 ssj0002769473 |
| Score | 2.3654985 |
| Snippet | Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid... Background Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular... BACKGROUND: Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular... Abstract Background Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on... |
| SourceID | doaj pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 39 |
| SubjectTerms | accounting Accumulation acetyl coenzyme A Algae alpha-glycerophosphoric acid Biodegradation Biodiesel fuels Biofuel Biofuels Biomass Biomass energy biomass production Biosynthesis biotechnology Carbohydrates Carbon dioxide Carbon dioxide fixation Carbon sequestration Cell culture Cell cycle Cell division Cellular manufacture Chlamydomonas Circadian rhythm Constraining Criminal investigation Cycles degradation dosage Fructose fructose 6-phosphate fuel production Gene expression Genomes Glucose glucose 6-phosphate Glycerol laboratories Laboratory equipment Light Light/dark cycling lighting Lipid Lipids Metabolic flux Metabolic profiling Metabolic rate Metabolism Metabolites Metabolomics Microalgae Monosaccharides Phosphate Phosphates photoperiod Photoperiodism Photoperiods Physiological aspects Pyruvic acid ribulose Salinity Substrates |
| SummonAdditionalLinks | – databaseName: Directory of Open Access Journals - May need to register for free articles dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LaxRBEG4kJz2Ib1ejtCIIwrDz6OcxBkMU9aAGcmv6mV3czCyZXcj-l_xYq2Zmlx2EePE6U9NMV31dD7r6a0LeSeedtqXKpJMhYzHXGQS9MoPUm0nOfMETHhT-9l2cnrEv5_x876ov7Anr6YF7xU2FdtEy53zwmhXRusKW2gshU8Wd5p33hZi3LaZ6H4ws43LYwyyUmLbgqQU2bWnk3MszNopCHVn_3y55LyaN-yX3AtDJA3J_yBzpUf_HD8mdWD8i9_b4BB-Tm69Yak-DvfpN_QYPPV5Qb9dtbCmSQW5ioIv5ch6o9X59OVzcRW0d6LzG7LEFgeWsWTVIf9yEzHVP8IQ-pNh0g81u1G1ot8OOY1_GFWBoMfc0LdbXMAhtFhGvPWrWLT2eAdg2oQGc25a2yyfk7OTTr-PTbLh-IfMy16ssVLoAE_MyOahiuVBVkilpj5RxWrhCg0FUHnKbpLe4m2vLyosANZHjhYq2ekoO6qaOzwmNVRlj4QoYAVKCKFUQLgmdNHeVYKGakHxrDuMHbnK8ImNhuhpFCdNb0IAFDVrQsAn5sPtk2RNz3Cb8EW28E0RO7e4BIM0MSDP_QtqEvEWEGGTNqLEt5wIM2JrPP3-YI64gky4gFZyQ94NQamAG3g6nHEAPSLQ1kjzcIs0MfqM1ED8qyRjPxYS82b2GFY_bOLaOYD6QURLqTpjjLTJMQ90Ivhb--lkP3t3kkaBIVQqUIkewHmln_KaezzrmcXDWVSnzF_9DnS_J3RIXJNIDFIfkYHW1jq8gwVu5191a_gP0M1GI priority: 102 providerName: Directory of Open Access Journals – databaseName: Scholars Portal Journals: Open Access dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9NAEF6VcoED4k2goAUhISGZ-rEP7wGhUlEVRDkAkXpb7ctJhGuHOJHq_8KPZcZxolhUPXG1xyvvvEez-w0hr6V1Vpk0j6SVPmIhVhEEvTSC1JtJzlzCC7wofPZNnI7Zl3N-vkc24616BjZXlnY4T2q8KN9d_m4_gMG_7ww-F4cN-GGBR7IUIurFEbtBbkJgkjjQ4IxtmwoIPS77xuaVnw1CU4fg_6-f3glUw0OUO1Hp5C6506eT9Ggt_3tkL1T3ye0dkMEH5M9XrL8PvVn8oq7Fm5AT6syqCQ1FhMg2eFrO5jNPjXOri36aFzWVp7MKU8oGCObTelkjJnLtI9s9wWv7kHfTFk_AUdvSru2Oa1-EJShWOXO0KFeXsAity4CzkOpVQ4-noIGtr0H5TUOb-UMyPvn08_g06mcyRE7Gahn5TCUgd54WFkpbLvKskEWhHOLIKWET5RTLYx-bQjqDLV6TZk54KJQsT_Jgskdkv6qr8ITQkKUhJDaBFSBPCDL3whZCFYrbTDCfjUi8EYd2PWA5zs0odVe45EKvJahBgholqNmIvN1-Ml-jdVxH_BFlvCVEoO3uQb2Y6N5utVA2GGat87CxJBibmFQ5IWSRcat4OiKvUEM0QmlUeFZnAgJs9Ocf3_URzyG9TiA_HJE3PVFRww6c6a8-AB8QfWtAebDRNL2xBQ1BJZOM8ViMyMvta3AD2NsxVQDxAU0uoRiFPV5DwxQUk-CA4a8fr5V3u3lELcqzHJgiB2o94M7wTTWbdnDk4MGzVMZP_wc7n5FbKRokYgYkB2R_uViF55D1Le2Lzpb_AgRFWGc priority: 102 providerName: Scholars Portal |
| Title | Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/30828384 https://www.proquest.com/docview/2193744506 https://www.proquest.com/docview/2187960130 https://www.proquest.com/docview/2498267942 https://pubmed.ncbi.nlm.nih.gov/PMC6383270 https://doaj.org/article/69bea4bbcdc941eab1a29c667f35b952 |
| Volume | 12 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9RAEF-0fdEH8dvTWlYRBCFcPjb78STX0lqLLdJauLdlv3I9vCZncwfe_-If60xuezYIfUkgmSzZndnZmZ3Z3xDyQVhnlcllIqzwCQupSmDRyxMwvZkomcvKCg8Kn5zyowt2PC7HccOtjWmVNzqxU9S-cbhHPoSZVQjGypR_nv9KsGoURldjCY37ZBuhy1CqxVhs9lhywRUTRQxmZpIPW1DZHLO3FILvpQnrLUcdav__uvnW4tRPnLy1Eh0-Jo-iCUlHa54_IfdC_ZQ8vAUs-Iz8-YY-99Cb65_UrfD044Q6s2xDSxEVchU8nU3nU0-Nc8urWMGLmtrTaY1mZAsE88tm0SAOcuMT2z3Bo_pga9MVZr1Ru6JdqB3bvgoLEKbZ1NFqtvwNjdBmFrD-UbNs6f4lSN3KNzCCpqXt_Dm5ODz4sX-UxDoMiROpWiS-UBnwuswrC-5syWVRiapSDrHjFLeZcorJ1KemEs5gWNfkheMenCNbZjKY4gXZqps6vCI0FHkImc2gBbANgpCe24qrSpW24MwXA5LesEO7CFKOtTJmunNWJNdrDmrgoEYOajYgnzafzNcIHXcR7yGPN4QIrt09aK4nOs5VzZUNhlnrPHQsC8ZmJleOc1EVpVVlPiDvUUI0wmfUmJ8zAQa2-uv5mR6VEkzqDGzCAfkYiaoGeuBMPO4A44CIWz3KnRtJ01GBtPqfuA_Iu81rmPoYzzF1APYBjRTggEIf76BhChxIULrw1y_XwrvpPCIVyULCoIieWPdGp_-mnl52EOSgtYtcpK_v_vU35EGOUw0RALIdsrW4Xoa3YMMt7G43UeEqD7_sku3R6Pj8GO57B6ffz3a7fRG4njD5FymYTlE |
| linkProvider | ProQuest |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELfGeAAeEN8UBhgEQkKKmsSOHT8gNAajZd0eYJP2ZvyVraJLytIK-r_wN_A3cpempRXS3vaaXCzbd76P3Pl3hLyU1lll0jySVvqIh1hFYPTSCFxvLjPukqzAi8L7B6J3xD8fZ8cb5M_iLgyWVS50YqOofeXwH3kXThaTnGexeDf-EWHXKMyuLlpozMViL8x-QshWv-1_AP6-StPdj4c7vajtKhA5GatJ5JlKYOZZWlgIzjKRs0IWhXKIhKaETZRTPI99bArpDCYpTcqc8ODq2yzJg2Ew7hVylTOw5HgzfffT8p9OKoXikrXJ0yQX3RpMhMBqMYVgf3HE18xf0yXgf1uwYgzXCzVXLN_uLXKzdVnp9lzGbpONUN4hN1aADO-S3wOM8bvenH-nboa3LU-oM9M61BRRKGfB09FwPPTUODc9azuGUVN6OizRba2BYHxaTSrEXa58ZJsnCA0Avj2dYZUdtTPapPZx7LMwAeEdDR0tRtNfMAitRgH7LVXTmu6cgpTPfAUcMzWtx_fI0aVw6D7ZLKsyPCQ0sDSExCYwAvgiQeZe2EKoQmWWCe5Zh8QLdmjXgqJjb46RboKjXOg5BzVwUCMHNe-QN8tPxnNEkIuI3yOPl4QI5t08qM5PdKsbtFA2GG6t87CwJBibmFQ5IWTBMquytENeoIRohOsosR7oBBhY6_7XL3o7y8GFT8AH7ZDXLVFRwQqcaa9XwD4gwtca5dZC0nSrsGr973h1yPPla1A1mD8yZQD2AU0uIeCFNV5AwxUErKDkYdYP5sK7XDwiI-Ush02Ra2K9tjvrb8rhaQN5DlaCpTJ-dPHUn5FrvcP9gR70D_Yek-spHjtEH0i2yObkfBqegP84sU-bQ0vJt8vWEn8BiumE4g |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Light%2Fdark+cycling+causes+delayed+lipid+accumulation+and+increased+photoperiod-based+biomass+yield+by+altering+metabolic+flux+in+oleaginous+Chlamydomonas+sp&rft.jtitle=Biotechnology+for+biofuels+and+bioproducts&rft.au=Kato%2C+Yuichi&rft.au=Fujihara%2C+Yusuke&rft.au=Vavricka%2C+Christopher+J&rft.au=Jo-Shu%2C+Chang&rft.date=2019-02-21&rft.pub=BioMed+Central&rft.eissn=2731-3654&rft.volume=12&rft_id=info:doi/10.1186%2Fs13068-019-1380-4 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1754-6834&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1754-6834&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1754-6834&client=summon |