Integrated pathway mining and selection of an artificial CYP79-mediated bypass to improve benzylisoquinoline alkaloid biosynthesis

• Published in: Microbial Cell Factories Vol. 23; no. 1; pp. 178 - 12
• Main Authors: Takenaka, Musashi, Kamasaka, Kouhei, Daryong, Kim, Tsuchikane, Keiko, Miyazawa, Seiha, Fujihana, Saeko, Hori, Yoshimi, Vavricka, Christopher J., Hosoyama, Akira, Kawasaki, Hiroko, Shirai, Tomokazu, Araki, Michihiro, Nakagawa, Akira, Minami, Hiromichi, Kondo, Akihiko, Hasunuma, Tomohisa
• Format: Journal Article
• Language: English
• Published: England Springer Science and Business Media LLC 15.06.2024; BioMed Central Ltd; BioMed Central; BMC
• Subjects: 3,4-dihydroxyphenylacetaldoxime; 3,4-Dihydroxyphenylacetic Acid - analogs & derivatives; 3,4-Dihydroxyphenylacetic Acid - metabolism; Alkaloids; Artificial metabolic pathway; Benzylisoquinoline alkaloid production; Benzylisoquinolines - metabolism; Biosynthesis; Biosynthetic Pathways; Computational enzyme mining; Computer Simulation; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Hydrogen peroxide; Metabolic Engineering - methods; Microbiological research; Microbiology; Mines and mineral resources; Monoamine oxidase; Physiological aspects; Plant metabolites; QR1-502; Resveratrol; Tetrahydropapaveroline - metabolism; Tyrosine; Tyrosine N-monooxygenase; Benzylisoquinoline alkaloid production; Computational enzyme mining; Tyrosine N-monooxygenase; Cytochrome P450; 3,4-dihydroxyphenylacetaldoxime; Artificial metabolic pathway

Computational mining of useful enzymes and biosynthesis pathways is a powerful strategy for metabolic engineering. Through systematic exploration of all conceivable combinations of enzyme reactions, including both known compounds and those inferred from the chemical structures of established reactio...