Broken replication forks trigger heritable DNA breaks in the terminus of a circular chromosome

• Published in: PLoS genetics Vol. 14; no. 3; p. e1007256
• Main Authors: Sinha, Anurag Kumar, Possoz, Christophe, Durand, Adeline, Desfontaines, Jean-Michel, Barre, François-Xavier, Leach, David R. F., Michel, Bénédicte
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.03.2018; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Cell Division; Chromosomes; Chromosomes, Bacterial; DNA Breaks, Double-Stranded; DNA Repair; DNA Replication; DNA, Bacterial - genetics; DNA, Circular - genetics; Escherichia coli - cytology; Escherichia coli - genetics; Escherichia coli Proteins - metabolism; Exodeoxyribonuclease V - metabolism; Genetic research; Life Sciences; Microscopy, Fluorescence; Models, Biological; Mutation; Properties

It was recently reported that the recBC mutants of Escherichia coli, deficient for DNA double-strand break (DSB) repair, have a decreased copy number of their terminus region. We previously showed that this deficit resulted from DNA loss after post-replicative breakage of one of the two sister-chrom...