SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells - Sasaki, Michihito; Uemura, Kentaro; Sato, Akihiko; Toba, Shinsuke; Sanaki, Takao; Maenaka, Katsumi; Hall, William W.; Orba, Yasuko; Sawa, Hirofumi (PLoS pathogens, 2021)
Keywords: ACE2; Amino Acid Sequence; Amino acids; Analysis; Angiotensin; Angiotensin-converting enzyme 2; Animals; Binding sites; Biology and life sciences; Caco-2 Cells; Cell Line; Chlorocebus aethiops; Cleavage; Cloning; Coronaviruses; COVID-19; Dilution; Gene mutations; Genetic aspects; Genomes; HEK293 Cells; Humans; Infections; Medicine and health sciences; Mutation; Nucleotides; Pandemics; Peptidyl-dipeptidase A; Progeny; Propagation; Proteins; Receptors; Research and Analysis Methods; S gene; SARS-CoV-2 - classification; SARS-CoV-2 - genetics; SARS-CoV-2 - growth & development; SARS-CoV-2 - physiology; Sequence Alignment; Serial Passage; Serine Endopeptidases - deficiency; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - genetics; Vero Cells; Viral diseases; Viral Tropism; Virions; Viruses; Japan