Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase

• Published in: Journal of molecular and cellular cardiology Vol. 92; pp. 30 - 40
• Main Authors: Tong, Xiaoyong, Khandelwal, Alok R., Wu, Xiaojuan, Xu, Zaicheng, Yu, Weimin, Chen, Caiyu, Zhao, Wanzhou, Yang, Jian, Qin, Zhexue, Weisbrod, Robert M., Seta, Francesca, Ago, Tetsuro, Lee, Kin Sing Stephen, Hammock, Bruce D., Sadoshima, Junichi, Cohen, Richard A., Zeng, Chunyu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2016
• Subjects: Animals; Aorta - metabolism; Aorta - pathology; Apolipoproteins E - genetics; Atherosclerosis; Atherosclerosis - genetics; Atherosclerosis - pathology; Blood Pressure - genetics; Cardiovascular; Cell Movement - genetics; Cell Proliferation - genetics; Gene Expression Regulation; Humans; Inflammation - genetics; Inflammation - pathology; Mice; Mice, Transgenic; Myocytes, Smooth Muscle - metabolism; Myocytes, Smooth Muscle - pathology; NADPH Oxidase 4; NADPH Oxidases - biosynthesis; NADPH Oxidases - genetics; Nox4; Reactive Oxygen Species - metabolism; RNA, Small Interfering - genetics; Smooth muscle; Soluble epoxide hydrolase 2; TNFα; TSP1; Smooth muscle; Nox4DN; H2O2; ORO; WD; ICAM1; DBP; MCP1; sEH; Atherosclerosis; ApoE; SDN; EETs; NTg; SBP; VCAM1; SMA; TPPU; SMC; siRNA; LCA; PWV; Soluble epoxide hydrolase 2; ND; ROS; Nox4; MAP; thrombospondin 1; intercellular adhesion molecule 1; systolic blood pressure; smooth muscle human Nox4DN transgenic mice; diastolic blood pressure; Oil Red O; small interference RNA; 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea; left common carotid artery; hydrogen peroxide; soluble epoxide hydrolase 2; H 2O 2; smooth muscle cells; ApoE deficient; Western diet; Nox4 dominant negative form P437H; pulse wave velocity; epoxyeicosatrienoic acids; smooth muscle alpha actin; tumor necrosis factor alpha; monocyte chemoattractant protein 1; non-transgenic littermate mice; reactive oxygen species; vascular cell adhesion molecule 1; mean arterial pressure; normal diet

Nox4-based NADPH oxidase is a major reactive oxygen species-generating enzyme in the vasculature, but its role in atherosclerosis remains controversial. Our goal was to investigate the role of smooth muscle Nox4 in atherosclerosis. Atherosclerosis-prone conditions (disturbed blood flow and Western d...