A system for the continuous directed evolution of biomolecules

• Published in: Nature (London) Vol. 472; no. 7344; pp. 499 - 503
• Main Authors: Esvelt, Kevin M., Carlson, Jacob C., Liu, David R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.04.2011; Nature Publishing Group
• Subjects: 631/181/2475; 631/181/735; Adenosine Triphosphate - metabolism; Bacteria; Bacteriology; Bacteriophage T3 - genetics; Bacteriophage T7 - enzymology; Bacteriophage T7 - genetics; Bacteriophages; Bacteriophages - enzymology; Bacteriophages - genetics; Bacteriophages - physiology; Binding sites; Biological and medical sciences; Cloning; Cytidine Triphosphate - metabolism; Directed Molecular Evolution - methods; Diverse techniques; DNA-Directed RNA Polymerases - biosynthesis; DNA-Directed RNA Polymerases - chemistry; DNA-Directed RNA Polymerases - genetics; DNA-Directed RNA Polymerases - metabolism; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - metabolism; Escherichia coli - virology; Evolution; Fundamental and applied biological sciences. Psychology; Gene mutations; Genetic aspects; Genetic engineering; Genetic transcription; Guanosine Triphosphate - metabolism; Humanities and Social Sciences; Identification and classification; letter; Molecular and cellular biology; multidisciplinary; Mutagenesis; Mutation; Physiological aspects; Plasmids; Promoter Regions, Genetic - genetics; Promoters (Genetics); Properties; Science; Science (multidisciplinary); Viral Proteins - biosynthesis; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; United States; Virus; Directed molecular evolution; Escherichia coli; Bacteria; Method; Gram negative bacteria; Enterobacteriaceae; Phage

Speedy route to new biomolecules Many biomolecules with useful properties have been generated by laboratory molecular evolution experiments, but the processes typically take days and require frequent human intervention. Esvelt et al . now describe a phage-assisted continuous evolution system that en...