Chromosomal location of the cadmium uptake gene (Cdu1) in durum wheat

• Published in: Genome Vol. 52; no. 9; pp. 741 - 747
• Main Authors: Knox, R.E, Pozniak, C.J, Clarke, F.R, Clarke, J.M, Houshmand, S, Singh, A.K
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.09.2009; Canadian Science Publishing NRC Research Press
• Subjects: bioaccumulation; Biological Transport; Cadmium; Cadmium - metabolism; cadmium uptake gene; chromosomal location; chromosome mapping; Chromosome Mapping - methods; Chromosomes; Chromosomes, Plant - genetics; cytogenetics; doubled haploid; doubled haploids; Durum wheat; Food products; Food safety; foods; genes; Genes, Plant - genetics; Genetic aspects; Genetic Markers; Genomics; Genotype & phenotype; Grain; haploïde doublé; heavy metal; Heavy metals; Lod Score; marker; marqueur; métal lourd; position chromosomique; Properties; Quantitative trait loci; Quantitative Trait Loci - genetics; screening; Seeds - metabolism; Species Specificity; toxic substances; translocation (plant physiology); Triticum - classification; Triticum - genetics; Triticum - metabolism; Triticum turgidum subsp. durum; uptake mechanisms; Canada
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/G09-042

Levels of the heavy metal cadmium (Cd) in food products are a food safety concern. Grain Cd is higher in durum (Triticum turgidum L. var. durum) than in common wheat, so reduction of Cd in durum grain is a priority of breeding programs. Previous research demonstrated that a single dominant gene, Cdu1, confers the low grain Cd phenotype, but the map location of the gene is not known. A doubled haploid population segregating for Cd concentration, developed from the cross of W9262-260D3 (a Kyle*2/Biodur inbred selection with low Cd uptake) and Kofa (high Cd uptake) and mapped with microsatellite markers, was used to locate Cdu1. Grain Cd concentration was determined by standard laboratory methods on field grain samples in 2000 and 2001. The Cd concentration segregated bimodally, allowing Cdu1 to be mapped qualitatively as well as quantitatively with quantitative trait locus analysis. The Cdu1 gene mapped to the long arm of chromosome 5B.