A VAMP-Binding Protein from Aplysia Required for Neurotransmitter Release
Before the fusion of synaptic vesicles with the plasma membrane, a protein complex is thought to form between VAMP--an integral membrane protein of the vesicle--and two proteins associated with the plasma membrane, SNAP-25 and syntaxin. The yeast two-hybrid interaction cloning system has now been us...
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Published in | Science (American Association for the Advancement of Science) Vol. 269; no. 5230; pp. 1580 - 1583 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for the Advancement of Science
15.09.1995
American Association for the Advancement of Science The American Association for the Advancement of Science |
Subjects | |
Online Access | Get full text |
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Summary: | Before the fusion of synaptic vesicles with the plasma membrane, a protein complex is thought to form between VAMP--an integral membrane protein of the vesicle--and two proteins associated with the plasma membrane, SNAP-25 and syntaxin. The yeast two-hybrid interaction cloning system has now been used to identify additional proteins from Aplysia that interact directly with VAMP. A 33-kilodalton membrane protein, termed VAP-33 (VAMP-associated protein of 33 kilodaltons), was identified whose corresponding messenger RNA was detected only in the central nervous system and the gill of Aplysia. Presynaptic injection of antibodies specific for VAP-33 inhibited synaptic transmission, which suggests that VAP-33 is required for the exocytosis of neurotransmitter. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0036-8075 1095-9203 |
DOI: | 10.1126/science.7667638 |