Genome-wide RNAi selection identifies a regulator of transmission stage-enriched gene families and cell-type differentiation in Trypanosoma brucei

• Published in: PLoS pathogens Vol. 13; no. 3; p. e1006279
• Main Authors: Rico, Eva, Ivens, Alasdair, Glover, Lucy, Horn, David, Matthews, Keith R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.03.2017; Public Library of Science (PLoS)
• Subjects: Animals; Biology; Biology and life sciences; Cell cycle; Cell Differentiation; Cell Differentiation - genetics; Cell fate; Differentiation (biology); Disease Models, Animal; Disease transmission; Enrichment; Evolution; Female; Flies; Flow Cytometry; Gene expression; Gene Expression Regulation; Gene Expression Regulation - genetics; Gene families; Genetic aspects; Genome-Wide Association Study; Genomes; Immunoblotting; Immunology; Infections; Life Sciences; Mammals; Medicine and Health Sciences; Messenger RNA; Mice; Midgut; Parasites; Proteins; Protozoa; Protozoan Proteins; Protozoan Proteins - biosynthesis; Protozoan Proteins - genetics; Repressors; RNA Interference; RNA-Binding Proteins; RNA-Binding Proteins - biosynthesis; RNA-Binding Proteins - genetics; RNA-mediated interference; Transcription; Transfection; Tropism; Trypanosoma brucei; Trypanosoma brucei brucei; Trypanosoma congolense; Trypanosoma vivax; Trypanosome; Trypanosomiasis, African; Trypanosomiasis, African - genetics; Trypanosomiasis, African - transmission; Scotland; Edinburgh Scotland

Trypanosoma brucei, causing African sleeping-sickness, exploits quorum-sensing (QS) to generate the 'stumpy forms' necessary for the parasite's transmission to tsetse-flies. These quiescent cells are generated by differentiation in the bloodstream from proliferative slender forms. Usi...