Reconstructing promoter activity from Lux bioluminescent reporters

• Published in: PLoS computational biology Vol. 13; no. 9; p. e1005731
• Main Authors: Iqbal, Mudassar, Doherty, Neil, Page, Anna M. L., Qazi, Saara N. A., Ajmera, Ishan, Lund, Peter A., Kypraios, Theodore, Scott, David J., Hill, Philip J., Stekel, Dov J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.09.2017; Public Library of Science (PLoS)
• Subjects: Bacteria; Bacterial Proteins - analysis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bayesian analysis; Biochemistry; Biology and Life Sciences; Bioluminescence; Biosensors; Biotechnology; Chemical synthesis; Computational Biology; Computer applications; Computer simulation; Councils; Destructive testing; E coli; Environmental science; Enzymes; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Euthanasia; Experimental data; Fatty acids; Food; Funding; Gene expression; Gene Expression - genetics; Genes, Reporter - genetics; Gram-positive bacteria; Health aspects; Light; Light levels; Luciferases - analysis; Luciferases - chemistry; Luciferases - genetics; Luciferases - metabolism; Luminescent Agents - analysis; Luminescent Agents - chemistry; Luminescent Agents - metabolism; Mathematical models; Molecular biology; Nonlinear Dynamics; Operons; Physical Sciences; Product inhibition; Promoter Regions, Genetic - genetics; Promoters (Genetics); Proteins; Research and Analysis Methods; Software; Spectrometry, Fluorescence; Staphylococcus aureus; Statistical inference; Supervision; Switching; Transcription

The bacterial Lux system is used as a gene expression reporter. It is fast, sensitive and non-destructive, enabling high frequency measurements. Originally developed for bacterial cells, it has also been adapted for eukaryotic cells, and can be used for whole cell biosensors, or in real time with li...