RESCRIPt: Reproducible sequence taxonomy reference database management

• Published in: PLoS computational biology Vol. 17; no. 11; p. e1009581
• Main Authors: Robeson, Michael S., O’Rourke, Devon R., Kaehler, Benjamin D., Ziemski, Michal, Dillon, Matthew R., Foster, Jeffrey T., Bokulich, Nicholas A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.11.2021; Public Library of Science (PLoS)
• Subjects: Animals; Biology and Life Sciences; Classification; Clustering; Computational Biology; Computer and Information Sciences; Database administration; Database Management Systems; Databases, Genetic - statistics & numerical data; Databases, Nucleic Acid; Deoxyribonucleic acid; Diet; DNA; DNA Barcoding, Taxonomic; DNA sequencing; Environmental DNA; Gene sequencing; Genes; Genetic engineering; Genomes; Genomics; Hepatitis; Humans; Metagenome; Metagenomics; Methods; Microbiomes; Microbiota - genetics; Nucleotide sequence; Nucleotide sequencing; Nucleotides; Phylogeny; Physical Sciences; Polls & surveys; Qualitative analysis; Quality standards; Reference materials; Reproducibility; Research and Analysis Methods; RNA, Ribosomal, 16S - genetics; Sequence Analysis; Software; Standardization; Taxonomy; United States

Nucleotide sequence and taxonomy reference databases are critical resources for widespread applications including marker-gene and metagenome sequencing for microbiome analysis, diet metabarcoding, and environmental DNA (eDNA) surveys. Reproducibly generating, managing, using, and evaluating nucleoti...