Knock-In Reporter Mice Demonstrate that DNA Repair by Non-homologous End Joining Declines with Age

• Published in: PLoS genetics Vol. 10; no. 7; p. e1004511
• Main Authors: Vaidya, Amita, Mao, Zhiyong, Tian, Xiao, Spencer, Brianna, Seluanov, Andrei, Gorbunova, Vera
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.07.2014; Public Library of Science (PLoS)
• Subjects: Age; Aging; Aging - genetics; Aging - pathology; Animals; Base Sequence; Biology and life sciences; Cancer; Cloning; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded; DNA damage; DNA Damage - genetics; DNA End-Joining Repair - genetics; DNA repair; DNA Repair - genetics; DNA-Binding Proteins - genetics; Efficiency; Fibroblasts; Gene Knock-In Techniques; Gene mutations; Genes, Reporter; Genetic research; Genomes; Green Fluorescent Proteins - genetics; Humans; Mice; Mutation; Proteins; Recombination, Genetic; Research and Analysis Methods; Skin; Software; Studies

Accumulation of genome rearrangements is a characteristic of aged tissues. Since genome rearrangements result from faulty repair of DNA double strand breaks (DSBs), we hypothesized that DNA DSB repair becomes less efficient with age. The Non-Homologous End Joining (NHEJ) pathway repairs a majority o...