Nicotiana Small RNA Sequences Support a Host Genome Origin of Cucumber Mosaic Virus Satellite RNA

• Published in: PLoS genetics Vol. 11; no. 1; p. e1004906
• Main Authors: Zahid, Kiran, Zhao, Jian-Hua, Smith, Neil A., Schumann, Ulrike, Fang, Yuan-Yuan, Dennis, Elizabeth S., Zhang, Ren, Guo, Hui-Shan, Wang, Ming-Bo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.01.2015; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Cucumber Mosaic Virus Satellite - genetics; Cucumovirus - genetics; Cucumovirus - pathogenicity; Deoxyribonucleic acid; Diseases and pests; DNA; DNA methylation; DNA Methylation - genetics; Gene Silencing; Genes; Genetic aspects; Genome, Plant; Genomes; Genomics; Glucuronidase - genetics; Health aspects; Helper Viruses - genetics; Hybridization; Methods; Nicotiana; Nicotiana - genetics; Nicotiana - virology; Plant viruses; Plant-pathogen relationships; Plants, Genetically Modified; RNA polymerase; RNA sequencing; RNA, Small Interfering; Satellites; Sequence Analysis, RNA; Transgenes; Viruses

Satellite RNAs (satRNAs) are small noncoding subviral RNA pathogens in plants that depend on helper viruses for replication and spread. Despite many decades of research, the origin of satRNAs remains unknown. In this study we show that a β-glucuronidase (GUS) transgene fused with a Cucumber mosaic v...