Autoselection of Cytoplasmic Yeast Virus Like Elements Encoding Toxin/Antitoxin Systems Involves a Nuclear Barrier for Immunity Gene Expression

• Published in: PLoS genetics Vol. 11; no. 5; p. e1005005
• Main Authors: Kast, Alene, Voges, Raphael, Schroth, Michael, Schaffrath, Raffael, Klassen, Roland, Meinhardt, Friedhelm
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.05.2015; Public Library of Science (PLoS)
• Subjects: Amino Acid Sequence; Cloning; Cloning, Molecular; Crystal structure; Cytoplasm - metabolism; Escherichia coli - genetics; Experiments; Gene expression; Gene Expression Regulation, Fungal; Genetic aspects; Identification and classification; Killer Factors, Yeast - genetics; Killer Factors, Yeast - metabolism; Kluyveromyces - genetics; Kluyveromyces - metabolism; Molecular Sequence Data; Open access publishing; Pichia - genetics; Pichia - metabolism; Plasmids; Proteins; Ribonucleases - genetics; Ribonucleases - metabolism; RNA, Fungal - genetics; Saccharomyces; Saccharomycetales - genetics; Saccharomycetales - metabolism; Yeast

Cytoplasmic virus like elements (VLEs) from Kluyveromyces lactis (Kl), Pichia acaciae (Pa) and Debaryomyces robertsiae (Dr) are extremely A/T-rich (>75%) and encode toxic anticodon nucleases (ACNases) along with specific immunity proteins. Here we show that nuclear, not cytoplasmic expression of...