Probing Xist RNA Structure in Cells Using Targeted Structure-Seq

• Published in: PLoS genetics Vol. 11; no. 12; p. e1005668
• Main Authors: Fang, Rui, Moss, Walter N., Rutenberg-Schoenberg, Michael, Simon, Matthew D.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.12.2015; Public Library of Science (PLoS)
• Subjects: Animals; Chromosomes; DNA methylation; DNA Methylation - genetics; Evolution, Molecular; Females; Gene expression; Humans; Methods; Mice; Nucleic Acid Conformation; Point Mutation - genetics; RNA Folding - genetics; RNA sequencing; RNA, Long Noncoding - genetics; RNA, Ribosomal - genetics; Sequence Alignment; Sequence Analysis, RNA; X Chromosome - genetics; X Chromosome Inactivation - genetics

The long non-coding RNA (lncRNA) Xist is a master regulator of X-chromosome inactivation in mammalian cells. Models for how Xist and other lncRNAs function depend on thermodynamically stable secondary and higher-order structures that RNAs can form in the context of a cell. Probing accessible RNA bas...