Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein

• Published in: Virology journal Vol. 18; no. 1; p. 16
• Main Authors: Tani, Hideki, Kimura, Miyuki, Tan, Long, Yoshida, Yoshihiro, Ozawa, Tatsuhiko, Kishi, Hiroyuki, Fukushi, Shuetsu, Saijo, Masayuki, Sano, Kaori, Suzuki, Tadaki, Kawasuji, Hitoshi, Ueno, Akitoshi, Miyajima, Yuki, Fukui, Yasutaka, Sakamaki, Ippei, Yamamoto, Yoshihiro, Morinaga, Yoshitomo
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.01.2021; BioMed Central; BMC
• Subjects: Animals; Antibodies; Antibodies, Neutralizing - blood; Antibodies, Neutralizing - immunology; Antibodies, Viral - blood; Antibodies, Viral - immunology; Antigens; biosafety; blood serum; Cell Line; Chemiluminescence; Cloning; Convalescence; Coronaviruses; COVID-19; COVID-19 - blood; COVID-19 - immunology; COVID-19 infection; COVID-19 Serological Testing - methods; Enzyme-linked immunosorbent assay; fluorescent antibody technique; Genetic aspects; Health aspects; Hospitalization; Humans; Immune response; Immunofluorescence; Infections; Inhibitory Concentration 50; luciferase; Luminescence; Lymphocytes; neutralization; Neutralization assay; neutralization tests; Neutralization Tests - methods; Observations; pathogens; Patients; Plasmids; Proteins; Pseudotyped virus; SARS-CoV-2; SARS-CoV-2 - genetics; SARS-CoV-2 - immunology; Sensitivity analysis; Serum; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - genetics; Spike Glycoprotein, Coronavirus - immunology; Spike protein; Stomatitis; Vesicular stomatitis Indiana virus - genetics; Vesiculovirus; Viral antibodies; Viral infections; Viral proteins; virology; Viruses; VSV; Whole blood; Japan; United States > US; Whole blood; VSV; Pseudotyped virus; Serum; Neutralization assay; SARS-CoV-2

SARS-CoV-2 is a novel coronavirus that emerged in 2019 and is now classified in the genus Coronavirus with closely related SARS-CoV. SARS-CoV-2 is highly pathogenic in humans and is classified as a biosafety level (BSL)-3 pathogen, which makes manipulating it relatively difficult due to its infectio...