Phenotypic characterization of lung macrophages in asthmatic patients: Overexpression of CCL17

• Published in: Journal of allergy and clinical immunology Vol. 130; no. 6; pp. 1404 - 1412.e7
• Main Authors: Staples, Karl J., Hinks, Timothy S.C., Ward, Jon A., Gunn, Victoria, Smith, Caroline, Djukanović, Ratko
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.12.2012; Elsevier; Elsevier Limited
• Subjects: 1-Phosphatidylinositol 3-kinase; adrenal cortex hormones; Adrenal Cortex Hormones - administration & dosage; Adult; Allergy and Immunology; Alveoli; Animal models; Asthma; Asthma - drug therapy; Asthma - immunology; Biological and medical sciences; Biomarkers; Biomarkers - metabolism; Bronchus; CCL17 protein; CCL22 protein; CD163 antigen; chemokine; chemokine CCL17; Chemokine CCL17 - genetics; Chemokine CCL17 - metabolism; chemokine CCL22; Chemokines; Chromones - pharmacology; Chronic obstructive pulmonary disease, asthma; Corticoids; Cytokines; disease severity; Drug therapy; enzyme inhibitors; Enzymes; Eosinophilia; Eosinophils - immunology; Female; Flow cytometry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene expression; gene expression regulation; gene overexpression; Genotype & phenotype; Helper cells; Humans; Immune system; Immunopathology; Inflammation; Interleukin 10; Ligands; Lung; Lymphocytes; Lymphocytes T; macrophage; Macrophages; Macrophages - drug effects; Macrophages - immunology; Male; Medical sciences; messenger RNA; Middle Aged; Monocytes; Morpholines - pharmacology; mRNA; patients; Phenotype; phosphatidylinositol 3-kinase; Phosphoinositide-3 Kinase Inhibitors; Pneumology; Proteins; Respiratory tract diseases; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Sputum; Sputum - drug effects; Sputum - immunology; steroid; Steroid hormones; steroids; Th2 Cells - immunology; Up-Regulation; Young Adult; AF647; chemokine; Arg1; M1; M2; CLEC10A; macrophage; FACS; PE-Cy7; BAL; Asthma; Lin; LDH; MMP; NF-κB; PE-AF610; APC; PI3K; PE; APC-Cy7; MDM; PerCP-Cy5.5; steroid; COPD; Matrix metalloproteinase; Alexa Fluor 647; Phycoerythrin–Alexa Fluor 610; Classically activated (macrophages); Bronchoalveolar lavage; Macrophage galactose C-type lectin (CD301); Allophycocyanin; Monocyte-derived macrophage; Chronic obstructive pulmonary disease; Nuclear factor κB; Arginase 1; Phycoerythrin–cyanine 7; Lineage; Lactate dehydrogenase; Alternatively activated (macrophages); Fluorescence-activated cell sorting; Peridinin chlorophyll protein–cyanine 5.5; Phycoerythrin; Phosphatidylinositol 3-kinase; Allophycocyanin–cyanine 7; Human; Steroid; Lung disease; Immunopathology; Respiratory disease; Lung; Gene overexpression; Respiratory system; Characterization; Chemokine; Phenotype; Immunology; Bronchus disease; Obstructive pulmonary disease; Macrophage

Studies with monocyte-derived macrophages (MDMs) and animal models have suggested a role for alternatively activated (M2) macrophages in asthmatic inflammation, but in vivo evidence for this phenotype in human asthma is lacking. To characterize the phenotype of lung macrophages from asthmatic patien...