Evaluation of RNAi and CRISPR technologies by large-scale gene expression profiling in the Connectivity Map

• Published in: PLoS biology Vol. 15; no. 11; p. e2003213
• Main Authors: Smith, Ian, Greenside, Peyton G., Natoli, Ted, Lahr, David L., Wadden, David, Tirosh, Itay, Narayan, Rajiv, Root, David E., Golub, Todd R., Subramanian, Aravind, Doench, John G.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.11.2017; Public Library of Science (PLoS)
• Subjects: Analysis; Authorship; Biology and life sciences; Cancer; Cell lines; Cells, Cultured; Clustered Regularly Interspaced Short Palindromic Repeats; Computer applications; CRISPR; DNA sequencing; Earth Sciences; Engineering and Technology; Funding; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Regulatory Networks - genetics; Genetic engineering; Genetic research; Genomes; Genomics - methods; Genomics - standards; Hep G2 Cells; HT29 Cells; Humans; Mammalian cells; MCF-7 Cells; Methods and Resources; MicroRNA; MicroRNAs; miRNA; Nucleotide sequencing; Physical Sciences; Principal components analysis; Reagents; Ribonucleic acid; RNA; RNA Interference - physiology; RNA, Small Interfering - genetics; RNA-mediated interference; Screens; Software; Studies; Supervision; Technology; Transcriptome

The application of RNA interference (RNAi) to mammalian cells has provided the means to perform phenotypic screens to determine the functions of genes. Although RNAi has revolutionized loss-of-function genetic experiments, it has been difficult to systematically assess the prevalence and consequence...