An Epigenetic Compound Library Screen Identifies BET Inhibitors That Promote HSV-1 and -2 Replication by Bridging P-TEFb to Viral Gene Promoters through BRD4

• Published in: PLoS pathogens Vol. 12; no. 10; p. e1005950
• Main Authors: Ren, Ke, Zhang, Wei, Chen, Xiaoqing, Ma, Yingyu, Dai, Yue, Fan, Yimei, Hou, Yayi, Tan, Ren Xiang, Li, Erguang
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2016; Public Library of Science (PLoS)
• Subjects: Academic libraries; Azepines - pharmacology; Biology and life sciences; Biotechnology; Blotting, Western; Care and treatment; Chromatin Immunoprecipitation; Colleges & universities; Deoxyribonucleic acid; DNA; Epigenesis, Genetic; Epigenetics; Funding; Gene expression; Gene Knockdown Techniques; Genetic aspects; Herpes Simplex; Herpesviridae; Herpesvirus 1, Human - drug effects; Herpesvirus 2, Human - drug effects; Herpesvirus infections; Host-Parasite Interactions - physiology; Humans; Infections; Laboratories; Medical schools; Medicine; Medicine and Health Sciences; Microscopy, Confocal; Nuclear Proteins - metabolism; Pathogens; Pharmaceuticals; Polymerase Chain Reaction; Positive Transcriptional Elongation Factor B - metabolism; Proteins; Research and Analysis Methods; Risk factors; RNA, Small Interfering; Transcription Factors - metabolism; Transfection; Triazoles - pharmacology; Virus Replication - drug effects; Virus Replication - physiology; Viruses

The human HSV-1 and -2 are common pathogens of human diseases. Both host and viral factors are involved in HSV lytic infection, although detailed mechanisms remain elusive. By screening a chemical library of epigenetic regulation, we identified bromodomain-containing protein 4 (BRD4) as a critical p...