CRISPR/Cas9-Mediated genomic knock out of tyrosine hydroxylase and yellow genes in cricket Gryllus bimaculatus

• Published in: PloS one Vol. 18; no. 4; p. e0284124
• Main Authors: Bai, Yun, He, Yuan, Shen, Chu-Ze, Li, Kai, Li, Dong-Liang, He, Zhu-Qing
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.04.2023; Public Library of Science (PLoS)
• Subjects: Animals; Biology and Life Sciences; Biosynthesis; Catecholamine; Catecholamines; Conversion; Crickets; CRISPR; CRISPR-Cas Systems - genetics; Defects; Dihydroxyphenylalanine; Dopachrome isomerase; Dopamine; Efficiency; Eggs; Embryogenesis; Embryonic growth stage; Enzymes; Evolution; Gene deletion; Gene regulation; Genes; Genetic aspects; Genetic regulation; Genetics; Genomes; Genomics; Gryllidae; Gryllus bimaculatus; Homozygotes; Humidity; Hybridization; Hydroxylases; Insects; Melanin; Melanins - genetics; Melanins - metabolism; Mutants; Mutation; Neurology; Nucleotides; Phenotypes; Physical Sciences; Physiological aspects; Physiology; Pigmentation; Proteins; Research and analysis methods; Tyrosine; Tyrosine 3-monooxygenase; Tyrosine 3-Monooxygenase - genetics; Tyrosine 3-Monooxygenase - metabolism; Wings; Y gene; Yellow protein; China; United States > US

Gryllus bimaculatus is an emerging model organism in various fields of biology such as behavior, neurology, physiology and genetics. Recently, application of reverse genetics provides an opportunity of understanding the functional genomics and manipulating gene regulation networks with specific phys...