Optimised protocol for monitoring SARS-CoV-2 in wastewater using reverse complement PCR-based whole-genome sequencing - Child, Harry T.; O’Neill, Paul A.; Moore, Karen; Rowe, William; Denise, Hubert; Bass, David; Wade, Matthew J.; Loose, Matt; Paterson, Steve; van Aerle, Ronny; Jeffries, Aaron R. (PloS one, 2023)
Keywords: Analysis; Animals; Asymptomatic; Biology and life sciences; Complement System Proteins; Coronaviruses; COVID-19; COVID-19 - diagnosis; COVID-19 - epidemiology; COVID-19 Testing; Data analysis; Disease transmission; DNA sequencing; Epidemics; Epidemiology; Gene sequencing; Genetic research; Genomes; Genomics; Health aspects; Health surveillance; Humans; Infections; Lab Protocol; Libraries; Medicine and health sciences; Monitoring; Next-generation sequencing; Nucleotide sequencing; Outbreaks; Pandemics; Pathogens; Polymerase Chain Reaction; Prevention; Process controls; Protocol; Public health; Purification; Quality control; Research and analysis methods; RNA; RNA, Viral - genetics; SARS-CoV-2 - genetics; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Sewage; Surveillance; Viral diseases; Viruses; Wastewater; Water sampling; Whole genome sequencing; United Kingdom; Netherlands; United Kingdom--UK; England