An Improved Method for Surface Immobilisation of RNA: Application to Small Non-Coding RNA - mRNA Pairing

• Published in: PloS one Vol. 8; no. 11; p. e79142
• Main Authors: Vincent, Helen A., Phillips, Jack O., Henderson, Charlotte A., Roberts, Adam J., Stone, Carlanne M., Mardle, Charlotte E., Butt, Louise E., Gowers, Darren M., Pickford, Andrew R., Callaghan, Anastasia J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.11.2013; Public Library of Science (PLoS)
• Subjects: Acids; Adenine; Binding; Binding sites; Biofilms; Biotin; Chemistry; Cholera toxin; Dengue fever; DNA Glycosylases - biosynthesis; DNA Glycosylases - genetics; E coli; Escherichia coli - chemistry; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli - pathogenicity; Gene expression; Gene regulation; Genes; Helicobacter pylori; Immobilization; Ligases; Mathematical analysis; Messenger RNA; Methods; Non-coding RNA; Nucleotides; Post-transcription; Purines; Ribonucleic acid; RNA; RNA ligase; RNA polymerase; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Messenger - chemistry; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Untranslated - chemistry; RNA, Small Untranslated - genetics; RNA, Small Untranslated - metabolism; RNA-protein interactions; Streptavidin; Surface Plasmon Resonance; Transcription (Genetics); Vibrio cholerae; Viral infections; Virulence; Waterborne diseases; United Kingdom > UK

Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the interactions of bacterial small non-coding RNA...