Comparative Analysis of Hemagglutination Inhibition Titers Generated Using Temporally Matched Serum and Plasma Samples

• Published in: PloS one Vol. 7; no. 12; p. e48229
• Main Authors: Defang, Gabriel N., Martin, Nicholas J., Burgess, Timothy H., Millar, Eugene V., Pecenka, LeNae A., Danko, Janine R., Arnold, John C., Kochel, Tadeusz J., Luke, Thomas C.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.12.2012; Public Library of Science (PLoS)
• Subjects: Antibodies; Antibodies, Viral - blood; Antibodies, Viral - immunology; Anticoagulants; Anticoagulants - pharmacology; Antigens; Biology; Biometrics; Blood & organ donations; Citrates - metabolism; Coefficient of variation; Comparative analysis; Correlation; Data collection; Enzymes; Health sciences; Hemagglutination; Hemagglutination inhibition; Hemagglutination Inhibition Tests - methods; Heparin - metabolism; HIV; Human immunodeficiency virus; Human subjects; Humans; Immunity; Immunogenicity; Immunoglobulins; Immunology; Infectious diseases; Influenza; Influenza A; Influenza A Virus, H1N1 Subtype - immunology; Influenza A Virus, H3N2 Subtype - immunology; Inhibition; Mathematical analysis; Medical research; Medicine; Plasma - drug effects; Plasma - metabolism; Plasma - virology; Preventive medicine; Seroconversion; Serum - drug effects; Serum - virology; Studies; Time Factors; Vaccines; Variability; Viruses; United States > US; Maryland

Influenza-specific hemaggluitination inhibition (HAI) antibody titer, an indicator of immunity to influenza, is often used to measure exposure to influenza in surveillance and immunogenicity studies. Traditionally, serum has been the specimen of choice for HAI assays, but a desire to reduce the amou...