Sensitivity of PCR Assays for Murine Gammaretroviruses and Mouse Contamination in Human Blood Samples

• Published in: PloS one Vol. 7; no. 5; p. e37482
• Main Authors: Lee, Li Ling, Lin, Lin, Bell, David S., Levine, Susan, Hanson, Maureen R.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.05.2012; Public Library of Science (PLoS)
• Subjects: Animals; Assaying; Biology; Blood; Carbon dioxide; Cell culture; Chronic fatigue syndrome; Contamination; Deoxyribonucleic acid; DNA; DNA Contamination; DNA, Viral - analysis; DNA, Viral - genetics; Experiments; Fatigue; Fibromyalgia; Gag protein; Gene sequencing; Humans; Identification methods; Laboratories; Leukemia; Leukemia Virus, Murine - genetics; Leukemia Virus, Murine - isolation & purification; Leukocytes (mononuclear); Medical research; Medicine; Mice; Molecular biology; Nucleic acids; Nucleotide sequence; Patients; Peripheral blood mononuclear cells; Polymerase chain reaction; Polymerase Chain Reaction - standards; Primers; Prostate cancer; Sensitivity and Specificity; Tumors; Viruses

Gammaretroviruses related to murine leukemia virus (MLV) have variously been reported to be present or absent in blood from chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) patients and healthy controls. Using subjects from New York State, we have investigated by PCR methods whether MLV-r...